SH-SY5Y-CAS9

location： Home > Products > Cas9 Stable Cell Lines > SH-SY5Y-CAS9

SH-SY5Y-CAS9 Order

Catalog#：YC-D014-Cas9-H

Price (USD)：-

Size： 1*10^6

Instruction：

SH-SY5Y Cell Line was generated by CRISPR-U™ gene-editing technology to stably express Cas9 protein.

Detailed Product Information Recommended Companion Products

Cell Info

Catalog	YC-D014-Cas9-H
Cell line	SH-SY5Y-CAS9	Cell Type	Human Choriocarcinoma Cell Line
Morphology	Epithelial-like, adherent	Passage ratio	1:2-1:3
Culture method	43.5%MEM+43.5%ham"s F-12+10%FBS+1%Glutamax+1%Sodium Pyruvate+1%NEAA
Cryopreservation solution	90%FBS+10%DMSO
Special Note	Cells require autocrine factors for survival, so do not passage the cells when the cell confluency is low, the suggested passage ratio is 1:2 and the time of each passaging is 48 hours. Pay attention that do not overgrow the cells, once cells are overgrown, the cell condition is unable to recover.

Product Validation Data

RT-QPCR

Sample Name	Target Name	Cт Mean	ΔCт
SH-SY5Y-CAS9	Cas9	21.61257362	6.61797619
SH-SY5Y-CAS9	h-actin-B	14.99459743	6.61797619
SH-SY5Y	Cas9	33.49401855	15.52625656
SH-SY5Y	h-actin-B	17.96776199	15.52625656

Cutting Efficiency Validation

Note: The above figure shows the sequencing peaks of the SH-SY5Y-CAS9 stable cell pool which is electroporated by SDHB gene targeting gRNA plasmid, after 48h antibiotic screening. The red arrow indicates the position where the nested peak appears, which shows that the genotype of the target site is significantly changed due to the cutting. Therefore, it indicates that Cas9 nuclease is successfully expressed.

Product Advantage

（1）Easy to use：Cas9 cell lines in our cell bank can stably express Cas9 protein. Each Cas9 Stable Cell Line is easy to use and enables gene knockout simply by transfecting gRNA, while transfection of gRNA and donor DNA results in gene knock-in or point mutations
（2）High-efficient KO：These Cas9 stable cell lines have been used to construct the KO cell lines for various genes, gene KO efficiency 5-10 times improved.
（3）High-quality cell：Selected cells, low passages, good cell condition, high activity, applicable for all kinds of gene-editing experiments.

Images

Ubigene