A549-EGFP cell line

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A549-EGFP Order

Catalog#

YC-C016-EGFP-P

Size

1*10^6

Transfection Culture Medium

Transfection Culture Medium(A549)

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Datasheet

Order

Detailed Product Information

Product category

EGFP Stable Cell Lines

cell name

A549-EGFP

Cell Morphology

Epithelial-like, adherent

Passage ratio

1:3~1:4

Species

Human

Reporter gene

EGFP

Resistance gene

puro

Construction method

Lentivirus

Mycoplasma test

negative

Culture method

90% F12K+10% FBS

Accession

CVCL_XB31(Cellosaurus)

STR Profile

Loci	STR Info (Sample Cell) Sample Cell Line:A549			STR Info (Cell bank) Cell Line:A-549
Loci	Allele1	Allele2	Allele3	Allele1	Allele2	Allele3
D5S818	11	11		11	11
D13S317	11	11		11	11
D7S820	8	11		8	11
D16S539	11	12		11	12
VWA	14	14		14	14
TH01	8	9.3		8	9.3
AMEL	X	Y		X	Y
TPOX	8	11		8	11
CSF1PO	10	12		10	12
D12S391	18	18
FGA	23	23
D2S1338	24	24
D21S11	29	29
D18S51	14	17
D8S1179	13	14
D3S1358	16	16
D6S1043	11	13
PENTAE	7	11
D19S433	13	13
PENTAD	9	9
D1S1656	17	18.3

* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

EGFP validation

Instruction

Use EGFP gene expressing lentivirus constructed by Ubigene to infect the A549 cell line， screen the cells based on the puromycin resistance marker and the optimal antibiotic screening concentration explored in advance, resulting in a A549-EGFP cell line that can maintain long-term, stable and high expression.

A549-EGFP

Catalog#

YC-C016-EGFP-P

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Order

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A549-EGFP cell line

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