GSDMD Knockout cell line (HTR-8/SVneo)

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GSDMD Knockout cell line (HTR-8/SVneo) Order

GSDMD Knockout cell line (HTR-8/SVneo)
Catalog#
YKO-H257
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
GSDMD Knockout cell line (HTR-8/SVneo)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
GSDMD knockout cell line (HTR-8/SVneo)
Cell Morphology
Endothelial cells, adherent
Passage ratio
1:2~1:4
Species
Human
Gene Name
GSDMD
Gene ID
79792
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
90%DMEM+10%FBS
Accession
CVCL_D9WW(Cellosaurus)
ExPASy Cellosaurus Database
Is a valuable online resource that compiles detailed information on over 100,000 cell lines from reputable cell banks such as ATCC, ECACC, and DSMZ. The database provides information including descriptions, origins, and characteristics of the cell lines, with broad reference value and scientific reliability. Additionally, the database provides information on cell line authentication, STR profiles, and potential contamination risks, helping researchers ensure the trustworthiness of the cell lines used in their experiments.
Target region
Knockout region: Exon 2 (based on transcript GSDMD-201)
Validation: PCR+Sanger Sequencing
GSDMD Knockout cell line (HTR-8/SVneo) Target region
STR Profile
Click to download the STR appraisal report
Loci STR Info (Sample Cell)
Sample Cell Line:HTR-8/SVneo		 STR Info (Cell bank)
Cell Line:HTR-8/SVneo
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 12121212
D13S317 912912
D7S820 12121212
D16S539 11131313
VWA 13181318
TH01 69.369.3
AMEL XXXX
TPOX 8888
CSF1PO 12121212
D12S391 1919
FGA 2123
D2S1338 2324
D21S11 2930
D18S51 1313
D8S1179 1515
D3S1358 1616
D6S1043 1419
PENTAE 77
D19S433 1415
PENTAD 912
D1S1656 1617.3
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the GSDMD gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the HTR-8/SVneo cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, GSDMD gene knockout GSDMD knockout cell line (HTR-8/SVneo) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
GSDMD Knockout cell line (HTR-8/SVneo)
GSDMD Knockout cell line (HTR-8/SVneo)
Catalog#
YKO-H257
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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