· Expression plasmid ·
The target coding sequence (CDS) was cloned into plasmid, and promoter on plasmid regulates the expression of the target gene. In addition, the reporter gene and selection marker can be used for screening. Ubigene developed a series of YOE plasmids, including lentivirus plasmids, AAV plasmids, and non-viral plasmids, which can be widely used in vitro or in vivo.
||Reporter gene; Selection marker
|Lentiviral YOE plasmid
|Non-viral YOE plasmid
|AAV YOE plasmid
Note:Ubigene provides customized plasmid solutions. In addition to the selection of the above-mentioned plasmid backbone, customers can also choose different promoters: constitutive/specific/inducible promoter, the selection of connection mode between multiple target genes, etc.
Restriction enzyme digestion and sequencing
Different species: mammals, zebrafish, primary cells, stem cells, cell lines, microorganisms, etc.
Different purposes: frame-shift mutation knockout, fragment knockout, precise knockout, point mutation, fragment knockin.