HGC-27-CAS9 cell line

location： Home > Products > Cas9 Stable Cell Lines > HGC-27-CAS9

HGC-27-CAS9 Order

Catalog#

YC-C079-Cas9-H

Size

1*10^6

Transfection Culture Medium

Transfection Culture medium-B

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Datasheet

Order

Detailed Product Information

Product category

Cas9 Stable Cell Lines

cell name

HGC-27-CAS9

Cell Morphology

Endothelial cells, adherent

Passage ratio

1:3~1:4

Species

Human

Expression gene

Cas9

Resistance gene

hygro

Construction method

Lentivirus

Mycoplasma test

negative

Culture method

90%RPMI-1640+10% FBS

Description

Use Cas9 gene expressing lentivirus constructed by Ubigene to infect the HGC-27 cell line， screen the cells based on the resistance marker and the optimal antibiotic screening concentration explored in advance, resulting in a HGC-27-CAS9 cell line that can maintain long-term, stable and high expression.

Product Validation Data（RT-QPCR）

Sample Name	Target Name	Cт Mean	ΔCт
HGC-27-CAS9	Cas9	19.70162010	5.17640495
HGC-27-CAS9	β-actin	14.52521515	5.17640495
HGC-27	Cas9	33.80643082	19.62654114
HGC-27	β-actin	14.17988968	19.62654114

Cutting Efficiency Validation

HGC-27-CAS9 cell line Product Validation Data（RT-QPCR）

Note: The above figure shows the sequencing peaks of the HGC-27-CAS9 stable cell pool which is electroporated by PIN1 gene targeting gRNA plasmid, after 48h antibiotic screening. The red arrow indicates the position where the nested peak appears, which shows that the genotype of the target site is significantly changed due to the cutting. Therefore, it indicates that Cas9 nuclease is successfully expressed.

STR Profile

Loci	STR Info (Sample Cell) Sample Cell Line:HGC-27			STR Info (Cell bank) Cell Line:HGC-27
Loci	Allele1	Allele2	Allele3	Allele1	Allele2	Allele3
D5S818	12	12		12	12
D13S317	10	11		10	11
D7S820	11	12	13.0	11	12	13.0
D16S539	10	11		10	11
VWA	14	14		14	14
TH01	9	9		9	9
AMEL	X	X		X	X
TPOX	8	8		8	8
CSF1PO	12	12		12	12
D12S391	20	21
FGA	22	22
D2S1338	22	24
D21S11	30	33	34.0
D18S51	16	17
D8S1179	11	11
D3S1358	17	17
D6S1043	18	18
PENTAE	18	18
D19S433	14	14
PENTAD	9	13
D1S1656	14	16

* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Product Advantage

Easy to use：Cas9 cell lines in our cell bank can stably express Cas9 protein. Each Cas9 Stable Cell Line is easy to use and enables gene knockout simply by transfecting gRNA, while transfection of gRNA and donor DNA results in gene knock-in or point mutations
01
High-efficient KO：These Cas9 stable cell lines have been used to construct the KO cell lines for various genes, gene KO efficiency 5-10 times improved.
02
High-quality cell：Selected cells, low passages, good cell condition, high activity, applicable for all kinds of gene-editing experiments.
03

HGC-27-CAS9

Catalog#

YC-C079-Cas9-H

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Order

Related Popular Products

KO Cell Lines

PIN1 Knockout cell line (HGC-27)
inquiry More details

Organism: Human

Gene Name: PIN1

Gene ID: 5300

Size: 1*10^6

Catalog#: YKO-H864

YKO-H864(HGC-27)
FGFR1 Knockout cell line(HGC-27)
inquiry More details

Organism: Human

Gene Name: FGFR1

Gene ID: 2260

Size: 1*10^6

Catalog#: YKO-H209

YKO-H209(HGC-27)

Luciferase Stable Cell Lines

HGC-27-Luc
inquiry More details

Catalog#: YC-C079-Luc-P

Size: 1*10^6

Instruction:

Wild-type Cell Lines

Human Gastric Cancer Cell Line(HGC-27)
inquiry More details

Catalog#: YC-C079

Size: 1*10^6

Instruction:

Related Popular Products

KO Cell Lines

PIN1 Knockout cell line (HGC-27)

Organism: Human

Gene Name: PIN1

Gene ID: 5300

Size: 1*10^6

Catalog#: YKO-H864

inquiry More details
FGFR1 Knockout cell line(HGC-27)

Organism: Human

Gene Name: FGFR1

Gene ID: 2260

Size: 1*10^6

Catalog#: YKO-H209

inquiry More details

Luciferase Stable Cell Lines

HGC-27-Luc

Price (USD): -

Catalog#: YC-C079-Luc-P

Size: 1*10^6

Instruction:

inquiry More details

Wild-type Cell Lines

Human Gastric Cancer Cell Line(HGC-27)

Price (USD): -

Catalog#: YC-C079

Size: 1*10^6

Instruction:

inquiry More details

Subscribe Us

* E-mail：

Country/Region：

Name：

By subscription, you consent to allow Ubigene Biosciences to store and process the information provided above to deliver the latest news, research spotlight, and promotions. You can unsubscribe from these communications at anytime.

HGC-27-CAS9 cell line

Ubigene