gene knockout project

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Red Cotton™ Gene Knockout Project

Based on our aim of "make genome editing easier!" and continuous innovation, Ubigene officially initiates the "Red Cotton™ Gene Knockout Project", relying on the improved CRISPR-U™ technology, Ubigene-developed Red Cotton™ CRISPR Gene Editing System, and High-throughput KO Plasmid Construction Platform.

This project was initiated to construct the most resource rich and well-established gene KO resource library worldwide, with the aim of providing superior resource sharing and more specialized technical services for basic research, drug R & D, disease diagnosis, treatment and evaluation in biomedical fields.

  •  KO Cell Line Bank
  •  gRNA Plasmid Bank
KO Cell Line Bank
5000+

We plan to expand the KO cell line resources of the KO cell line bank to 5000 within next 3-5 years.

2021
Over 600 in-stock KO cells
2022
Over 2200 in-stock KO cells
2023
Over 3600 in-stock KO cells
Before  2024
Over 5000 in-stock KO cells

The construction of KO cell line bank is a key project of Ubigene's "Red Cotton™ Gene Knockout Project". At present, we have preliminarily constructed a KO cell line bank, with more than 600 KO cell lines in-stock covering 10 research fields; We plan to expand the KO cell line resources of the KO cell line bank to 5000 within next 3-5 years.

To enable the KO Cell Line Bank has more promising applications, Ubigene combined bioinformatics technology with big data analysis technology to sort the cell lines and genes, and construct the customized KO cell line project with two main lines --signaling pathways and disease types:

Main Line 1
Signaling pathway research
8 popular signaling pathwaysnearly 900 genesover 1700 KO cell lines

In order to facilitate the research of signaling pathways, Ubigene has screened 8 popular signaling pathways including NF-kB, Hedgehog, JAK-STAT, MAPK, Notch, PI3K-Akt, TFG-beta, and Wnt and their relevant key genes (over 900 genes). We plan to construct more than 1700 KO cell lines.

Signaling pathway

  • NF-kB
  • Hedgehog
  • JAK-TAT
  • MAPK
  • Notch
  • PI3K-Akt
  • TFG-beta
  • Wnt

Note: the gene list would be appropriately updated according to the research progress and research focus in each field.

(Slide down for more)

Main Line 2
Popular cells and genes
over 2700 KO cellsnearly 900 genescovering 100 diseases

In order to help with the research of disease discovery and treatment, we have screened 10 types of popular cell lines and nearly 900 genes, covering 8 human systems and nearly 100 diseases, and we plan to construct more than 2700 KO cell lines.

System  
  • Mental diseases
  • Endocrine diseases
  • Immune diseases
  • Infectious diseases
  • Genetic diseases
  • Cardiovascular diseases
  • Ear diseases
  • Bone diseases
  • Cancer diseases
  • Respiratory diseases
  • Reproductive diseases
  • Blood diseases
  • Metabolic diseases
Disease Type
Cell Line  
  • HCT 116
  • THP-1
  • RAW 264.7
  • HepG2
  • Hela
  • 4T1
  • Huh7
  • MC3T3
  • MDA-MB-231
  • U-87MG
Gene
Gene ID

Note: the gene list would be appropriately updated according to the research progress and research focus in each field.

(Slide down for more)

gRNA Plasmid Bank
25,000+

scale of KO cell bank to achieve the goal of >25000 gRNA plasmids within next 3-5 years.

2021
Over 10000 gRNA plasmids in-stock
2022
Over 15000 gRNA plasmids in-stock
2023
Over 20000 gRNA plasmids in-stock
Before  2024
Over 25000 gRNA plasmids in-stock

As the basis of the "Red Cotton™ Gene Knockout Project", the Red Cotton™ gRNA Plasmid Bank has more than 10000 gRNA KO plasmids, and will continue to expand the scale of KO cell bank to achieve the goal of >25000 gRNA plasmids within next 3-5 years.

Based on our CRISPR-U™ Technology and thousands of gene-editing success cases, Ubigene has successfully developed a series of gRNA plasmids with high gene-editing efficiency. Ubigene's plasmids can enter cells via regular transfection methods and can edit target sites efficiently after transient expression of high levels of Cas9 protein in target cells. Also, our plasmids can be co-transfected with a variety of gRNAs to target multiple sites in cells, which can be widely used in different fields of gene-editing research.

Ubigene, make genome editing easier

  • Red Cotton™ CRISPR Gene Editing Designer

    Red Cotton™ CRISPR Gene Editing Designer

    Ubigene's Red Cotton™CRISPR Gene Editing Designer has been used more than 40000 times, which successfully designed more than 30000 CRISPR gene KO strategies, and our Red Cotton™CRISPR Gene Editing Designer has successfully solved problems for thousands of researchers.

    Click here to get more information >
  • CRISPR-U™ technology

    CRISPR-U™ Technology

    Ubigene has successfully modified over 5000 genes from more than 100 cell lines with our developed CRISPR-U™ technology, and the KO efficiency leaps to 80%, and has higher homologous recombination efficiency. It can easily complete KO, point mutation and KI.

    Click here to get more information >
  • EZ-editor™ Gene-editing Products

    EZ-editor™ Gene-editing Products

    With 12 years of experience, Ubigene has exclusively innovated and developed 6 product lines, including Monoclone Validation Kit (extraction free), Wild-type Cell Lines, Stable Cell Lines (Cas9, Luciferase), Transfection Culture Medium, Over 10,000 in-stock gRNA plasmids, Cas9/GFP/mCherry/Luciferase expression Lentivirus, Mycoplasma Treatment Kit, which fully covers the experiment process of gene-editing in order to simplify the experiment process and improve the efficiency of gene-editing. Make genome editing easier!

    Click here to get more information >
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