Knockdown Plasmid
RNA interference (RNAi) is induced by a highly conserved, double-stranded RNA (dsRNA), which would cause specific degradation of homologous mRNA. Specific genes can be silenced, resulting in the loss of function or suppression of gene expression.
| YSH series | Vector | Reporter gene; Selection marker |
|---|
| Lentiviral YSH plasmid | YOE-LV001 | EGFP/mCherry; Puro/Neo |
| Non-viral YSH plasmid | YSH-RP001 | EGFP/mCherry; Puro/Neo |
| AAV YSH plasmid | YSH-AVV001 | EGFP/mCherry |
Note: Ubigene provides customized plasmid solutions, including different types of backbone, expression system, shRNA design, and Pol II promoters (Mir30-shRNA), etc.
Validation
Restriction enzyme digestion and sequencing
Application
Different species: mammals, zebrafish, primary cells, stem cells, cell lines, microorganisms, etc.
Different purposes: frame-shift mutation knockout, fragment knockout, precise knockout, point mutation, fragment knockin.
