CRISPR library is a high-throughput gene screening method established based on CRISPR/Cas9 technology. It identifies phenotype-related genes or screens new drug targets through functional screening, enrichment and deep sequencing analysis. The scope of screening can be the whole genome, a certain gene family, or a certain signal pathway gene. CRISPR library screening has become the preferred platform for large-scale gene function screening benefited from the characteristics of CRISPR/Cas9 such as versatility, low noise, high knockout efficiency, and less off-target effect.
Focusing on gene editing and having rich experience in cell line gene editing, Ubigene can provide 8 major libraries and one-stop services including sgRNA library construction, virus packaging, cell transfection, antibiotic screening, NGS sequencing, and data analysis.
Ubigene provides one-stop services for CRISPR-KO, CRISPRa and CRISPRi customized libraries, including sgRNA library construction, virus packaging, cell transfection, antibiotic screening, high-throughput sequencing and data analysis. We offer multiple deliverables meet different research needs.
Including gRNA design, chip synthesis, plasmid construction, plasmid extraction and NGS validation (coverage>99%, uniformity < 10)
Plasmid (100μg, transfection ready) NGS validation report
If a pre-made CRISPR plasmid library is provided, we can amplify the library, transform the plasmid to E. coli (coverage>100X), provide NGS validation (coverage 100X-500X), and then deliver the amplified plasmid library for downstream screening.
Small pack (100μg, transfection ready) Large pack (100μg, transfection ready) NGS validation report
3rd generation lentiviral packaging system, highly secure. Lentivirus titer ≥1x10^8 TU/ml guaranteed.
Small pack (1x10^8 TU lentiviral particles) Large pack (5x10^8 TU lentiviral particles) Lentivirus titer test report.
Infect cells with lentivirus library (MOI<0.3, only one virus transduce into one cell), and then conduct antibiotic screening according to the resistance gene carried on the plasmid to generate a cell pool.
Cell pool & NGS analysis report
Perform cell screening with the compound provided by customers, collect baseline/NC/sample, and extract DNA for NGS and gRNA difference analysis.
Cell pool & NGS analysis report
Ubigene's off-shelf whole genome CRISPR library of human and mouse can achieve high throughput and rapid screening of functional genes of the entire genome. The human whole genome CRISPR libraries include the single plasmid system (gRNA and Cas9 are in the same plasmid) and the dual plasmid system (gRNA and Cas9 are in different plasmids). At present, only single plasmid system of the mouse whole genome CRISPR library is provided. These three libraries are divided into two sub-libraries, A and B. Each library has designed three gRNAs for each gene, and also contains 1000 control gRNAs for non targeted genomes. Among them, library A has designed four gRNAs for each miRNA. The two sub-libraries can be used separately or together.
To screen the genes necessary for tumor growth and find new targets for tumor therapy.
To screen gene pairs with synthetic lethality for tumor therapy
To screen the genes causing drug resistance of tumors and study the mechanism of drug resistance
To screen host factors related to viral infection, including receptor and endoplasmic reticulum function related genes
Use CPRIPR library to validate the specificity of antibody
Use CPRIPR library to study gene function and its mechanism in signal pathway
As low as $900, high titer and viability, efficiently transfect each cell!
Over a hundred types of cell lines, stably express Cas9, easily achieve gene editing by tranfecting gRNA and donor DNA
The 3rd generation packaging system, optimal ratio of different helper plasmids, improve lentivirus yield!