Hep G2-CAS9 cell line

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Hep G2-CAS9 Order

Catalog#

YC-C001-Cas9-H

Size

1*10^6

Transfection Culture Medium

Transfection Culture medium-A

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Datasheet

Order

Detailed Product Information

Product category

Cas9 Stable Cell Lines

cell name

Hep G2-CAS9

Cell Morphology

Epithelial-like, adherent

Passage ratio

1:2~1:4

Species

Human

Expression gene

Cas9

Resistance gene

hygro

Construction method

Lentivirus

Mycoplasma test

negative

Culture method

90% DMEM+10% FBS

Description

Use Cas9 gene expressing lentivirus constructed by Ubigene to infect the Hep G2 cell line， screen the cells based on the resistance marker and the optimal antibiotic screening concentration explored in advance, resulting in a Hep G2-CAS9 cell line that can maintain long-term, stable and high expression.

Product Validation Data（RT-QPCR）

Sample Name	Target Name	Cт Mean	ΔCт
Hep G2-CAS9	Cas9	15.44887924	1.67105198
Hep G2-CAS9	β-actin	13.77782726	1.67105198
Hep G2	Cas9	36.25628281	22.33372402
Hep G2	β-actin	13.92255878	22.33372402

Cutting Efficiency Validation

Hep G2-CAS9 cell line Product Validation Data（RT-QPCR）

Note: The above figure shows the sequencing peaks of the Hep G2-CAS9 stable cell pool which is electroporated by CGAS gene targeting gRNA plasmid, after 48h antibiotic screening. The red arrow indicates the position where the nested peak appears, which shows that the genotype of the target site is significantly changed due to the cutting. Therefore, it indicates that Cas9 nuclease is successfully expressed.

STR Profile

Loci	STR Info (Sample Cell) Sample Cell Line:Hep G2			STR Info (Cell bank) Cell Line:GS-HepG2
Loci	Allele1	Allele2	Allele3	Allele1	Allele2	Allele3
D5S818	11	12		11	12
D13S317	9	13		9	13
D7S820	10	10		10	10
D16S539	12	12		12	12
VWA	17	17		17	17
TH01	9	9		9	9
AMEL	X	Y		X	Y
TPOX	8	9		8	9
CSF1PO	10	11		10	11
D12S391	21	25
FGA	22	25
D2S1338	19	20
D21S11	29	31
D18S51	13	14
D8S1179	15	16
D3S1358	15	16
D6S1043	13	13
PENTAE	15	20
D19S433	15.2	15.2
PENTAD	9	13
D1S1656	11	12

* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Product Advantage

Easy to use：Cas9 cell lines in our cell bank can stably express Cas9 protein. Each Cas9 Stable Cell Line is easy to use and enables gene knockout simply by transfecting gRNA, while transfection of gRNA and donor DNA results in gene knock-in or point mutations
01
High-efficient KO：These Cas9 stable cell lines have been used to construct the KO cell lines for various genes, gene KO efficiency 5-10 times improved.
02
High-quality cell：Selected cells, low passages, good cell condition, high activity, applicable for all kinds of gene-editing experiments.
03

Hep G2-CAS9

Catalog#

YC-C001-Cas9-H

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Order

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Hep G2-CAS9 cell line

Ubigene