ALKBH8 Knockout cell line (Calu-3)

ALKBH8 Knockout cell line (Calu-3) Order

Catalog#

YKO-H1146

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Order

Detailed Product Information

Product category

KO Cell Lines

cell name

ALKBH8 Knockout cell line（Calu-3）

Cell Morphology

Epithelial-like, adherent

Passage ratio

1：2-1：3

Species

Human

Gene Name

ALKBH8

Gene ID

91801

Gene Information

NCBI

Construction method

Electroporation/Lentivirus

Mycoplasma test

negative

Culture method

90% MEM+10%FBS

Target region

Knockout region: Exon 3 (based on transcript ALKBH8-205)
Validation: PCR+Sanger Sequencing

STR Profile

Loci	STR Info (Sample Cell) Sample Cell Line:Calu-3			STR Info (Cell bank) Cell Line:Calu-3
Loci	Allele1	Allele2	Allele3	Allele1	Allele2	Allele3
D5S818	11	11		11	11
D13S317	12	12		12	12
D7S820	10	11		10	11
D16S539	12	14		12	14
VWA	16	17		16	17
TH01	6	9.3		6	9.3
AMEL	X	X		X	X
TPOX	8	8		8	8
CSF1PO	11	12		11	12
D12S391	20	21	22.0
FGA	25	25
D2S1338	19	19
D21S11	28	30
D18S51	17	17
D8S1179	11	15
D3S1358	15	18
D6S1043	11	11
PENTAE	5	21
D19S433	12	13
PENTAD	9	16
D1S1656	12	15.3

* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the ALKBH8 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the Calu-3 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, ALKBH8 gene knockout ALKBH8 Knockout cell line（Calu-3） will be expanded and cryopreserved.

Knockout Cell Line Work Flow and Validatio

ALKBH8 Knockout cell line (Calu-3)

Catalog#

YKO-H1146

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Order

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