ANO1 Knockout cell line (A549)

ANO1 Knockout cell line (A549) Order

Catalog#

YKO-HS13461

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Order

Detailed Product Information

Product category

KO Cell Lines

cell name

ANO1 Knockout cell line (A549)

Cell Morphology

Epithelial-like, adherent

Passage ratio

1:3~1:4

Species

Human

Gene Name

ANO1

Gene ID

55107

Gene Information

NCBI

Construction method

Electroporation/Lentivirus

Mycoplasma test

negative

Culture method

90% F12K+10% FBS

Target region

Knockout region: Exon 4 (based on transcript ANO1-202)
Validation: PCR+Sanger Sequencing

STR Profile

Loci	STR Info (Sample Cell) Sample Cell Line:A549			STR Info (Cell bank) Cell Line:A-549
Loci	Allele1	Allele2	Allele3	Allele1	Allele2	Allele3
D5S818	11	11		11	11
D13S317	11	11		11	11
D7S820	8	11		8	11
D16S539	11	12		11	12
VWA	14	14		14	14
TH01	8	9.3		8	9.3
AMEL	X	Y		X	Y
TPOX	8	11		8	11
CSF1PO	10	12		10	12
D12S391	18	18
FGA	23	23
D2S1338	24	24
D21S11	29	29
D18S51	14	17
D8S1179	13	14
D3S1358	16	16
D6S1043	11	13
PENTAE	7	11
D19S433	13	13
PENTAD	9	9
D1S1656	17	18.3

* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the ANO1 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the A549 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, ANO1 gene knockout ANO1 Knockout cell line (A549) will be expanded and cryopreserved.

Knockout Cell Line Work Flow and Validatio

ANO1 Knockout cell line (A549)

Catalog#

YKO-HS13461

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Order

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