BCAT1 Knockout cell line (Huh-7)

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BCAT1 Knockout cell line (Huh-7) Order

BCAT1 Knockout cell line (Huh-7)
Catalog#
YKO-H418
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
BCAT1 Knockout cell line (Huh-7)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
BCAT1 Knockout cell line (Huh-7)
Morphology
Epithelial-like, adherent
Passage ratio
1:2~1:3
Species
Human
Gene Name
BCAT1
Gene ID
586
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
90%DMEM+10% FBS
Accession
CVCL_E0VG(Cellosaurus)
ExPASy Cellosaurus Database
Is a valuable online resource that compiles detailed information on over 100,000 cell lines from reputable cell banks such as ATCC, ECACC, and DSMZ. The database provides information including descriptions, origins, and characteristics of the cell lines, with broad reference value and scientific reliability. Additionally, the database provides information on cell line authentication, STR profiles, and potential contamination risks, helping researchers ensure the trustworthiness of the cell lines used in their experiments.
Target region
Knockout region: Exon 3 (based on transcript BCAT1-201)
Validation: PCR+Sanger Sequencing
BCAT1 Knockout cell line (Huh-7) Target region
STR Profile
Click to download the STR appraisal report
Loci STR Info (Sample Cell)
Sample Cell Line:Huh-7		 STR Info (Cell bank)
Cell Line:HuH-7
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 12121212
D13S317 10111011
D7S820 11111111
D16S539 10101010
VWA 16181618
TH01 7777
AMEL XXXX
TPOX 811811
CSF1PO 11111111
D12S391 2021
FGA 2223
D2S1338 1919
D21S11 3030
D18S51 1515
D8S1179 1414
D3S1358 1515
D6S1043 1315
PENTAE 1111
D19S433 1314
PENTAD 1212
D1S1656 1616
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the BCAT1 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the Huh-7 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, BCAT1 gene knockout BCAT1 Knockout cell line (Huh-7) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
BCAT1 Knockout cell line (Huh-7)
BCAT1 Knockout cell line (Huh-7)
Catalog#
YKO-H418
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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