Cd36 Knockout cell line (RAW264.7)

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Cd36 Knockout cell line (RAW264.7) Order

Cd36 Knockout cell line (RAW264.7)
Catalog#
YKO-M244
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Cd36 Knockout cell line (RAW264.7)
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Detailed Product Information

Product category
KO Cell Lines
cell name
Cd36 Knockout cell line (RAW264.7)
Species
Mouse
Gene Name
Cd36
Gene ID
12491
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Target region
Knockout region: Exon 3 (based on transcript Cd36-204)
Validation: PCR+Sanger Sequencing
Cd36 Knockout cell line (RAW264.7) Target region

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the Cd36 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the RAW264.7 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, Cd36 gene knockout Cd36 Knockout cell line (RAW264.7) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
Cd36 Knockout cell line (RAW264.7)
Cd36 Knockout cell line (RAW264.7)
Catalog#
YKO-M244
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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