EMP1 Knockout cell line (T24)

EMP1 Knockout cell line (T24) Order

Catalog#

YKO-H1150

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Order

Detailed Product Information

Product category

KO Cell Lines

cell name

EMP1 Knockout cell line（T24）

Cell Morphology

Epithelial-like, adherent

Passage ratio

1:3~1:4

Species

Human

Gene Name

EMP1

Gene ID

2012

Gene Information

NCBI

Construction method

Electroporation/Lentivirus

Mycoplasma test

negative

Culture method

90%RPMI-1640+10%FBS

Target region

Knockout region: Exon 3,Exon 4 (based on transcript EMP1-201)
Validation: PCR+Sanger Sequencing

STR Profile

Loci	STR Info (Sample Cell) Sample Cell Line:T24			STR Info (Cell bank) Cell Line:T24
Loci	Allele1	Allele2	Allele3	Allele1	Allele2	Allele3
D5S818	10	12		10	12
D13S317	12	12		12	12
D7S820	10	11		10	11
D16S539	9	9		9	9
VWA	17	17		17	17
TH01	6	6		6	6
AMEL	X	X		X	X
TPOX	8	11		8	11
CSF1PO	10	12		10	12
D12S391	18	18
FGA	17	22
D2S1338	20	23
D21S11	29	29
D18S51	16	18
D8S1179	14	14
D3S1358	16	16
D6S1043	11	11
PENTAE	7	10
D19S433	14	14
PENTAD	11	15
D1S1656	12	15

* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the EMP1 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the T24 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, EMP1 gene knockout EMP1 Knockout cell line（T24） will be expanded and cryopreserved.

Knockout Cell Line Work Flow and Validatio

EMP1 Knockout cell line (T24)

Catalog#

YKO-H1150

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Order

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