FTO Knockout cell line (Vips)

FTO Knockout cell line (Vips) Order

Catalog#

YKO-H728

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Order

Detailed Product Information

Product category

KO Cell Lines

cell name

FTO Knockout cell line (Vips)

Species

Human

Gene Name

FTO

Gene ID

79068

Gene Information

NCBI

Construction method

Electroporation/Lentivirus

Mycoplasma test

negative

Target region

Knockout region: Exon 3 (based on transcript FTO-206)
Validation: PCR+Sanger Sequencing

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the FTO gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the Vips cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, FTO gene knockout FTO Knockout cell line (Vips) will be expanded and cryopreserved.

Knockout Cell Line Work Flow and Validatio

FTO Knockout cell line (Vips)

Catalog#

YKO-H728

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Order

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FTO Knockout cell line (Vips)

Ubigene