HMGB1 Knockout cell line（LS 174T）

HMGB1 Knockout cell line（LS 174T） Order

Catalog#

YKO-H913

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Order

Detailed Product Information

Product category

KO Cell Lines

cell name

HMGB1 Knockout cell line（LS 174T）

Morphology

Epithelial-like,adherent

Passage ratio

1:2~1:4

Species

Human

Gene Name

HMGB1

Gene ID

3146

Gene Information

NCBI

Construction method

Electroporation/Lentivirus

Mycoplasma test

negative

Culture method

90%DMEM+10% FBS

Target region

Knockout region: Exon 3 (based on transcript HMGB1-203)
Validation: PCR+Sanger Sequencing

STR Profile

Loci	STR Info (Sample Cell) Sample Cell Line:LS 174T			STR Info (Cell bank) Cell Line:LS174T
Loci	Allele1	Allele2	Allele3	Allele1	Allele2	Allele3
D5S818	11	16		11	14	15.0
D13S317	10	10		10	10
D7S820	11	11		11	11
D16S539	11	13		11	13
VWA	15	16	17.0	15	17	18.0
TH01	6	7		6	7
AMEL	X	X		X	X
TPOX	8	9		8	9
CSF1PO	10	13		10	14
D12S391	16	20
FGA	20	21	22.0
D2S1338	18	22
D21S11	29	30	31.0
D18S51	11	13
D8S1179	12	16
D3S1358	15	17
D6S1043	12	13	14.0
PENTAE	15	16
D19S433	14	15
PENTAD	10	10
D1S1656	12	13	18.3

* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the HMGB1 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the LS 174T cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, HMGB1 gene knockout HMGB1 Knockout cell line（LS 174T） will be expanded and cryopreserved.

Knockout Cell Line Work Flow and Validatio

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