SGK1 Knockout cell line (SU-DHL-4)

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SGK1 Knockout cell line (SU-DHL-4) Order

SGK1 Knockout cell line (SU-DHL-4)
Catalog#
YKO-H188
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
SGK1 Knockout cell line (SU-DHL-4)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
SGK1 Knockout cell line (SU-DHL-4)
Cell Morphology
Lymphoblast,suspension
Passage ratio
1：2-1：3
Species
Human
Gene Name
SGK1
Gene ID
6446
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
90%RPMI-1640+10% FBS
Accession
CVCL_E1CE(Cellosaurus)
ExPASy Cellosaurus Database
Is a valuable online resource that compiles detailed information on over 100,000 cell lines from reputable cell banks such as ATCC, ECACC, and DSMZ. The database provides information including descriptions, origins, and characteristics of the cell lines, with broad reference value and scientific reliability. Additionally, the database provides information on cell line authentication, STR profiles, and potential contamination risks, helping researchers ensure the trustworthiness of the cell lines used in their experiments.
Target region
Knockout region: Exon 4,Exon 5,Exon 6,Exon 7,Exon 8,Exon 9,Exon 10,Exon 11,Exon 12,Exon 13,Exon 14 (based on transcript SGK1-204)
Validation: PCR+Sanger Sequencing
SGK1 Knockout cell line (SU-DHL-4) Target region
STR Profile
Click to download the STR appraisal report
Loci STR Info (Sample Cell)
Sample Cell Line:SU-DHL-4		 STR Info (Cell bank)
Cell Line:SU-DHL-4
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 11121112
D13S317 11121112
D7S820 811811
D16S539 11131113
VWA 18191819
TH01 69.369.3
AMEL XYXY
TPOX 911911
CSF1PO 12121212
D12S391 1820
FGA 1921
D2S1338 1719
D21S11 2930
D18S51 1416
D8S1179 1313
D3S1358 1516
D6S1043 1111
PENTAE 711
D19S433 1415.2
PENTAD 914
D1S1656 1518.3
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the SGK1 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the SU-DHL-4 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, SGK1 gene knockout SGK1 Knockout cell line (SU-DHL-4) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
SGK1 Knockout cell line (SU-DHL-4)
SGK1 Knockout cell line (SU-DHL-4)
Catalog#
YKO-H188
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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