SNORA59B Knockout cell line (SK-MES-1)

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SNORA59B Knockout cell line (SK-MES-1) Order

Catalog#

YKO-H165

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Order

Detailed Product Information

Product category

KO Cell Lines

cell name

SNORA59B Knockout cell line (SK-MES-1)

Cell Morphology

Epithelial-like, adherent

Passage ratio

1:3~1:4

Species

Human

Gene Name

SNORA59B

Gene ID

677882

Gene Information

NCBI

Construction method

Electroporation/Lentivirus

Mycoplasma test

negative

Culture method

90%MEM+10% FBS

Accession

CVCL_E1C9(Cellosaurus)

STR Profile

Loci	STR Info (Sample Cell) Sample Cell Line:SK-MES-1			STR Info (Cell bank) Cell Line:SK-MES-1
Loci	Allele1	Allele2	Allele3	Allele1	Allele2	Allele3
D5S818	11	11		11	11
D13S317	11	11		11	11
D7S820	8	8		8	8
D16S539	13	13		13	13
VWA	14	14		14	14
TH01	6	9.3		6	9.3
AMEL	X	Y		X	Y
TPOX	8	8		8	8
CSF1PO	12	12		12	12
D12S391	22	22
FGA	20	24
D2S1338	19	19
D21S11	29	30
D18S51	17	17
D8S1179	13	14
D3S1358	16	16
D6S1043	12	19
PENTAE	5	11
D19S433	14	14
PENTAD	12	13
D1S1656	17.3	18.3

* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the SNORA59B gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the SK-MES-1 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, SNORA59B gene knockout SNORA59B Knockout cell line (SK-MES-1) will be expanded and cryopreserved.

Knockout Cell Line Work Flow and Validatio

SNORA59B Knockout cell line (SK-MES-1)

Catalog#

YKO-H165

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Order

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SNORA59B Knockout cell line (SK-MES-1)

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