SQSTM1 Knockout cell line(BEAS-2B)

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SQSTM1 Knockout cell line(BEAS-2B) Order

SQSTM1 Knockout cell line(BEAS-2B)
Catalog#
YKO-H152
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
SQSTM1 Knockout cell line(BEAS-2B)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
SQSTM1 Knockout cell line (BEAS-2B)
Morphology
Epithelial-like, adherent
Passage ratio
1:3~1:4
Species
Human
Gene Name
SQSTM1
Gene ID
8878
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
90% DMEM+10% FBS
Accession
CVCL_D8EQ(Cellosaurus)
ExPASy Cellosaurus Database
Is a valuable online resource that compiles detailed information on over 100,000 cell lines from reputable cell banks such as ATCC, ECACC, and DSMZ. The database provides information including descriptions, origins, and characteristics of the cell lines, with broad reference value and scientific reliability. Additionally, the database provides information on cell line authentication, STR profiles, and potential contamination risks, helping researchers ensure the trustworthiness of the cell lines used in their experiments.
Target region
Knockout region: Exon 3 (based on transcript SQSTM1-202)
Validation: PCR+Sanger Sequencing
SQSTM1 Knockout cell line(BEAS-2B) Target region
STR Profile
Click to download the STR appraisal report
Loci STR Info (Sample Cell)
Sample Cell Line:BEAS-2B		 STR Info (Cell bank)
Cell Line:BEAS-2B
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 12131213
D13S317 13131313
D7S820 10131013
D16S539 12121212
VWA 17181718
TH01 79.379.3
AMEL XYXY
TPOX 611611
CSF1PO 912912
D12S391 1718
FGA 2024
D2S1338 2223
D21S11 2830
D18S51 1819
D8S1179 1315
D3S1358 1517
D6S1043 1218
PENTAE 58
D19S433 13.215.2
PENTAD 313
D1S1656 1414
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the SQSTM1 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the BEAS-2B cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, SQSTM1 gene knockout SQSTM1 Knockout cell line (BEAS-2B) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
SQSTM1 Knockout cell line(BEAS-2B)
SQSTM1 Knockout cell line(BEAS-2B)
Catalog#
YKO-H152
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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