TLR3 Knockout cell line (A-431)

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TLR3 Knockout cell line (A-431) Order

TLR3 Knockout cell line (A-431)
Catalog#
YKO-H072
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
TLR3 Knockout cell line (A-431)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
TLR3 Knockout cell line (A-431)
Cell Morphology
Epithelial-like, adherent
Passage ratio
1:3~1:5
Species
Human
Gene Name
TLR3
Gene ID
7098
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
88% DMEM+10% FBS+1%Glutamax+1%Sodium Pyruvate
Accession
CVCL_D7JA(Cellosaurus)
ExPASy Cellosaurus Database
Is a valuable online resource that compiles detailed information on over 100,000 cell lines from reputable cell banks such as ATCC, ECACC, and DSMZ. The database provides information including descriptions, origins, and characteristics of the cell lines, with broad reference value and scientific reliability. Additionally, the database provides information on cell line authentication, STR profiles, and potential contamination risks, helping researchers ensure the trustworthiness of the cell lines used in their experiments.
Target region
Knockout region: Exon 2 (based on transcript TLR3-201)
Validation: PCR+Sanger Sequencing
TLR3 Knockout cell line (A-431) Target region
STR Profile
Click to download the STR appraisal report
Loci STR Info (Sample Cell)
Sample Cell Line:A-431		 STR Info (Cell bank)
Cell Line:A-431
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 12131213
D13S317 913913
D7S820 10101010
D16S539 12141214
VWA 15171517
TH01 9999
AMEL XXXX
TPOX 11111111
CSF1PO 11121112
D12S391 1823
FGA 2020
D2S1338 1720
D21S11 2828
D18S51 1317
D8S1179 1313
D3S1358 1414
D6S1043 1218
PENTAE 1213
D19S433 1515.2
PENTAD 1111
D1S1656 1617
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the TLR3 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the A-431 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, TLR3 gene knockout TLR3 Knockout cell line (A-431) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
TLR3 Knockout cell line (A-431)
TLR3 Knockout cell line (A-431)
Catalog#
YKO-H072
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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