ITCH Knockout cell line (HeLa)

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ITCH Knockout cell line (HeLa) Order

ITCH Knockout cell line (HeLa)
Catalog#
YKO-H615
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
ITCH Knockout cell line (HeLa)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
ITCH Knockout cell line (HeLa)
Cell Morphology
Epithelial-like, adherent
Passage ratio
1：2-1：4
Species
Human
Gene Name
ITCH
Gene ID
83737
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
90%DMEM+10% FBS
Accession
CVCL_E0FE(Cellosaurus)
ExPASy Cellosaurus Database
Is a valuable online resource that compiles detailed information on over 100,000 cell lines from reputable cell banks such as ATCC, ECACC, and DSMZ. The database provides information including descriptions, origins, and characteristics of the cell lines, with broad reference value and scientific reliability. Additionally, the database provides information on cell line authentication, STR profiles, and potential contamination risks, helping researchers ensure the trustworthiness of the cell lines used in their experiments.
Target region
Knockout region: Exon 8 (based on transcript ITCH-202)
Validation: PCR+Sanger Sequencing
ITCH Knockout cell line (HeLa) Target region
STR Profile
Click to download the STR appraisal report
Loci STR Info (Sample Cell)
Sample Cell Line:HeLa		 STR Info (Cell bank)
Cell Line:HELA
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 11121112
D13S317 1213.31213.3
D7S820 812812
D16S539 910910
VWA 16181618
TH01 7777
AMEL XXXX
TPOX 812812
CSF1PO 910910
D12S391 202425.0
FGA 2121
D2S1338 1717
D21S11 2728
D18S51 1616
D8S1179 1213
D3S1358 1518
D6S1043 1819
PENTAE 717
D19S433 1314
PENTAD 815
D1S1656 1215
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the ITCH gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the HeLa cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, ITCH gene knockout ITCH Knockout cell line (HeLa) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
ITCH Knockout cell line (HeLa)
ITCH Knockout cell line (HeLa)
Catalog#
YKO-H615
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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