JUN Knockout cell line (HCT 116)

JUN Knockout cell line (HCT 116) Order

Catalog#

YKO-HS2731

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Order

Detailed Product Information

Product category

KO Cell Lines

cell name

JUN Knockout cell line (HCT 116)

Morphology

Epithelial-like, adherent

Passage ratio

1:2~1:4

Species

Human

Gene Name

JUN

Gene ID

3725

Gene Information

NCBI

Construction method

Electroporation/Lentivirus

Mycoplasma test

negative

Culture method

90%McCOY's 5A+10% FBS

Target region

Knockout region: Exon 1 (based on transcript JUN-201)
Validation: PCR+Sanger Sequencing

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the JUN gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the HCT 116 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, JUN gene knockout JUN Knockout cell line (HCT 116) will be expanded and cryopreserved.

Knockout Cell Line Work Flow and Validatio

JUN Knockout cell line (HCT 116)

Catalog#

YKO-HS2731

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Order

Related Popular Products

Wild-type Cell Lines

Human Colon Carcinoma Cell line(HCT 116)
inquiry More details

Catalog#: YC-C004

Size: 1*10^6

Instruction:

Gene Knockout kit

EZ-editor™Gene Knockout kit
inquiry More details

Catalog#: MY-001

Size: -

Instruction:

EZ-cryo™ cell freezing medium

EZ-cryo™ Cell Freezing Medium
inquiry More details

Catalog#: YK-CR-50;YK-CR-100

Size: 50ml;100ml

Instruction:

KO Cell Lines

JUN Knockout cell line (HeLa)
inquiry More details

Organism: Human

Gene Name: JUN

Gene ID: 3725

Size: 1*10^6

Catalog#: YKO-HS3854

YKO-HS3854(HeLa)
JUN Knockout cell line (HEK293)
inquiry More details

Organism: Human

Gene Name: JUN

Gene ID: 3725

Size: 1*10^6

Catalog#: YKO-HS1240

YKO-HS1240(HEK293)
JUN Knockout cell line (A549)
inquiry More details

Organism: Human

Gene Name: JUN

Gene ID: 3725

Size: 1*10^6

Catalog#: YKO-HS483

YKO-HS483(A549)

Related Popular Products

Wild-type Cell Lines

Human Colon Carcinoma Cell line(HCT 116)

Price (USD): -

Catalog#: YC-C004

Size: 1*10^6

Instruction:

inquiry More details

Gene Knockout kit

EZ-editor™Gene Knockout kit

Price (USD): 998

Catalog#: MY-001

Size: -

Instruction:

inquiry More details

EZ-cryo™ cell freezing medium

EZ-cryo™ Cell Freezing Medium

Price (USD): -;-

Catalog#: YK-CR-50;YK-CR-100

Size: 50ml;100ml

Instruction:

inquiry More details

KO Cell Lines

JUN Knockout cell line (HeLa)

Organism: Human

Gene Name: JUN

Gene ID: 3725

Size: 1*10^6

Catalog#: YKO-HS3854

inquiry More details
JUN Knockout cell line (HEK293)

Organism: Human

Gene Name: JUN

Gene ID: 3725

Size: 1*10^6

Catalog#: YKO-HS1240

inquiry More details
JUN Knockout cell line (A549)

Organism: Human

Gene Name: JUN

Gene ID: 3725

Size: 1*10^6

Catalog#: YKO-HS483

inquiry More details

JUN Knockout cell line (HCT 116)

Ubigene