LC12A9 Knockout cell line (COS-1)

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LC12A9 Knockout cell line (COS-1) Order

Catalog#

YKO-R074

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Order

Detailed Product Information

Product category

KO Cell Lines

cell name

LC12A9 Knockout cell line (COS-1)

Cell Morphology

Epithelial-like, adherent

Passage ratio

1:3-1:4

Species

Green monkey

Gene Name

LC12A9

Gene ID

103246714

Gene Information

NCBI

Construction method

Electroporation/Lentivirus

Mycoplasma test

negative

Culture method

90%DMEM+10%FBS

Target region

Knockout region: Exon 4 (based on transcript SLC12A9-201)
Validation: PCR+Sanger Sequencing

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the LC12A9 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the COS-1 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, LC12A9 gene knockout LC12A9 Knockout cell line (COS-1) will be expanded and cryopreserved.

Knockout Cell Line Work Flow and Validatio

LC12A9 Knockout cell line (COS-1)

Catalog#

YKO-R074

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Order

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LC12A9 Knockout cell line (COS-1)

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