MACF1 Knockout cell line (Hep G2)

MACF1 Knockout cell line (Hep G2) Order

Catalog#

YKO-HT1827

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Order

Detailed Product Information

Product category

KO Cell Lines

cell name

MACF1 Knockout cell line (Hep G2)

Species

Human

Gene Name

MACF1

Gene ID

23499

Gene Information

NCBI

Construction method

Electroporation/Lentivirus

Mycoplasma test

negative

Target region

Knockout region: Exon 2 (based on transcript MACF1-230)
Validation: PCR+Sanger Sequencing

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the MACF1 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the Hep G2 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, MACF1 gene knockout MACF1 Knockout cell line (Hep G2) will be expanded and cryopreserved.

Knockout Cell Line Work Flow and Validatio

MACF1 Knockout cell line (Hep G2)

Catalog#

YKO-HT1827

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Order

Related Popular Products

Wild-type Cell Lines

Human Hepatoma Cell Line(Hep G2)
inquiry More details

Catalog#: YC-C001

Size: 1*10^6

Instruction:

Gene Knockout kit

EZ-editor™Gene Knockout kit
inquiry More details

Catalog#: MY-001

Size: -

Instruction:

EZ-cryo™ cell freezing medium

EZ-cryo™ Cell Freezing Medium
inquiry More details

Catalog#: YK-CR-50;YK-CR-100

Size: 50ml;100ml

Instruction:

KO Cell Lines

Macf1 Knockout cell line (MC3T3-E1)
inquiry More details

Organism: Mouse

Gene Name: Macf1

Gene ID: 11426

Size: 1*10^6

Catalog#: YKO-M236

YKO-M236(MC3T3-E1 Subclone 14)
MACF1 Knockout cell line (HeLa)
inquiry More details

Organism: Human

Gene Name: MACF1

Gene ID: 23499

Size: 1*10^6

Catalog#: YKO-HS08371

YKO-HS08371(HeLa)
MACF1 Knockout cell line (HCT 116)
inquiry More details

Organism: Human

Gene Name: MACF1

Gene ID: 23499

Size: 1*10^6

Catalog#: YKO-HS08370

YKO-HS08370(HCT 116)

Related Popular Products

Wild-type Cell Lines

Human Hepatoma Cell Line(Hep G2)

Price (USD): -

Catalog#: YC-C001

Size: 1*10^6

Instruction:

inquiry More details

Gene Knockout kit

EZ-editor™Gene Knockout kit

Price (USD): 998

Catalog#: MY-001

Size: -

Instruction:

inquiry More details

EZ-cryo™ cell freezing medium

EZ-cryo™ Cell Freezing Medium

Price (USD): -;-

Catalog#: YK-CR-50;YK-CR-100

Size: 50ml;100ml

Instruction:

inquiry More details

KO Cell Lines

Macf1 Knockout cell line (MC3T3-E1)

Organism: Mouse

Gene Name: Macf1

Gene ID: 11426

Size: 1*10^6

Catalog#: YKO-M236

inquiry More details
MACF1 Knockout cell line (HeLa)

Organism: Human

Gene Name: MACF1

Gene ID: 23499

Size: 1*10^6

Catalog#: YKO-HS08371

inquiry More details
MACF1 Knockout cell line (HCT 116)

Organism: Human

Gene Name: MACF1

Gene ID: 23499

Size: 1*10^6

Catalog#: YKO-HS08370

inquiry More details

MACF1 Knockout cell line (Hep G2)

Ubigene