MCOLN1 Knockout cell line (T98G)

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MCOLN1 Knockout cell line (T98G) Order

Catalog#

YKO-H792

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Order

Detailed Product Information

Product category

KO Cell Lines

cell name

MCOLN1 Knockout cell line（T98G）

Cell Morphology

Fibroblast，adherent

Passage ratio

1:2-1:4

Species

Human

Gene Name

MCOLN1

Gene ID

57192

Gene Information

NCBI

Construction method

Electroporation/Lentivirus

Mycoplasma test

negative

Culture method

88%MEM+10%FBS+1%NEAA+1%Sodium Pyruvate 100 mM Solution

Accession

CVCL_E1CJ(Cellosaurus)

STR Profile

Loci	STR Info (Sample Cell) Sample Cell Line:T98G			STR Info (Cell bank) Cell Line:T98G [T98-G]
Loci	Allele1	Allele2	Allele3	Allele1	Allele2	Allele3
D5S818	10	12		10	12
D13S317	13	13		13	13
D7S820	9	10		9	10
D16S539	13	13		13	13
VWA	17	20		17	20
TH01	7	9.3		7	9.3
AMEL	X	Y		X	Y
TPOX	8	8		8	8
CSF1PO	10	12		10	12
D12S391	17	18
FGA	21	22
D2S1338	19	24
D21S11	28	32.2
D18S51	13	16
D8S1179	13	14
D3S1358	16	16
D6S1043	14	14
PENTAE	16	16
D19S433	12	13
PENTAD	10	11
D1S1656	14	17

* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the MCOLN1 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the T98G cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, MCOLN1 gene knockout MCOLN1 Knockout cell line（T98G） will be expanded and cryopreserved.

Knockout Cell Line Work Flow and Validatio

MCOLN1 Knockout cell line (T98G)

Catalog#

YKO-H792

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Order

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MCOLN1 Knockout cell line (T98G)

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