NFE2L2 Knockout cell line (U-251 MG)

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NFE2L2 Knockout cell line (U-251 MG) Order

NFE2L2 Knockout cell line (U-251 MG)
Catalog#
YKO-H924
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
NFE2L2 Knockout cell line (U-251 MG)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
NFE2L2 Knockout cell line（U251MG）
Cell Morphology
Epithelial-like, adherent
Passage ratio
1:2~1:4
Species
Human
Gene Name
NFE2L2
Gene ID
4780
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
90% DMEM+10%FBS
Accession
CVCL_E1E3(Cellosaurus)
ExPASy Cellosaurus Database
Is a valuable online resource that compiles detailed information on over 100,000 cell lines from reputable cell banks such as ATCC, ECACC, and DSMZ. The database provides information including descriptions, origins, and characteristics of the cell lines, with broad reference value and scientific reliability. Additionally, the database provides information on cell line authentication, STR profiles, and potential contamination risks, helping researchers ensure the trustworthiness of the cell lines used in their experiments.
Target region
Knockout region: Exon 2 (based on transcript NFE2L2-201)
Validation: PCR+Sanger Sequencing
NFE2L2 Knockout cell line (U-251 MG) Target region
STR Profile
Click to download the STR appraisal report
Loci STR Info (Sample Cell)
Sample Cell Line:U251MG		 STR Info (Cell bank)
Cell Line:KNS-89
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 11121112
D13S317 10111011
D7S820 10121012
D16S539 12121212
VWA 16181618
TH01 9.39.39.39.3
AMEL XXXX
TPOX 8888
CSF1PO 11121112
D12S391 1722
FGA 2125
D2S1338 2424
D21S11 2929
D18S51 1313
D8S1179 1315
D3S1358 1616
D6S1043 1212
PENTAE 710
D19S433 1315
PENTAD 1212
D1S1656 1617.3
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the NFE2L2 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the U251MG cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, NFE2L2 gene knockout NFE2L2 Knockout cell line（U251MG） will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
NFE2L2 Knockout cell line (U-251 MG)
NFE2L2 Knockout cell line (U-251 MG)
Catalog#
YKO-H924
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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