NOTUM Knockout cell line (Hep G2)

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NOTUM Knockout cell line (Hep G2) Order

NOTUM Knockout cell line (Hep G2)
Catalog#
YKO-HT4755
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
NOTUM Knockout cell line (Hep G2)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
NOTUM Knockout cell line (Hep G2)
Species
Human
Gene Name
NOTUM
Gene ID
147111
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Target region
Knockout region: Exon 4 (based on transcript NOTUM-201)
Validation: PCR+Sanger Sequencing
NOTUM Knockout cell line (Hep G2) Target region

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the NOTUM gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the Hep G2 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, NOTUM gene knockout NOTUM Knockout cell line (Hep G2) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
NOTUM Knockout cell line (Hep G2)
NOTUM Knockout cell line (Hep G2)
Catalog#
YKO-HT4755
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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  • NOTUM Knockout cell line (HeLa)

    NOTUM Knockout cell line (HeLa)

    Organism: Human
    Gene Name: NOTUM
    Gene ID: 147111
    Size: 1*10^6
    Catalog#: YKO-HS11728
      inquiry   More details
  • NOTUM Knockout cell line (HCT 116)

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    Gene Name: NOTUM
    Gene ID: 147111
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