SIGLEC7 Knockout cell line(H1)

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SIGLEC7 Knockout cell line(H1) Order

Catalog#

YKO-H359

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Order

Detailed Product Information

Product category

KO Cell Lines

cell name

SIGLEC7 Knockout cell line (H1)

Morphology

Spherical clone

Passage ratio

1:6-1:8

Species

Human

Gene Name

SIGLEC7

Gene ID

27036

Gene Information

NCBI

Construction method

Electroporation/Lentivirus

Mycoplasma test

negative

Culture method

EZ-Stem™ Cell Culture Medium

Target region

Knockout region: Exon 1 (based on transcript SIGLEC7-202)
Validation: PCR+Sanger Sequencing

STR Profile

Loci	STR Info (Sample Cell) Sample Cell Line:H1			STR Info (Cell bank) Cell Line:WAe001-A-16
Loci	Allele1	Allele2	Allele3	Allele1	Allele2	Allele3
D5S818	9	11		9	11
D13S317	8	11		8	11
D7S820	8	12		8	12
D16S539	9	13		9	13
VWA	15	17		15	17
TH01	9.3	9.3		9.3	9.3
AMEL	X	Y		X	Y
TPOX	8	11		8	11
CSF1PO	12	13		12	13
D12S391	20	20
FGA	20	24
D2S1338	18	19
D21S11	28	32.2
D18S51	17	18
D8S1179	12	13
D3S1358	15	15
D6S1043	12	14
PENTAE	10	12
D19S433	13	15
PENTAD	10	13
D1S1656	16	17.3

* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the SIGLEC7 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the H1 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, SIGLEC7 gene knockout SIGLEC7 Knockout cell line (H1) will be expanded and cryopreserved.

Knockout Cell Line Work Flow and Validatio

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SIGLEC7 Knockout cell line(H1)

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