What is the trick of generating a stable cell line that match our research needs? That is to ensure the stability of cell condition during every step of experiments, especially when the target gene is large and the cell line itself is difficult to culture or transfect. Ubigene has explored the best MOI as well as culture system for each cell line from our cell bank, so as to ensure that the constructed stable cell line can continuously express/knockdown the target gene!
Reliable cell lines
Low passage, high viability
Best infection and culture system
specific transfection culture medium for each cell line
MOI database
Tested the best MOI of every cell line
Optimized single cell cloning system
Cell Monoclonal Culture Medium improves colony formation rate up to 30%
| Type | Overexpression | Knockdown (1 shRNA) |
Deliverables | ||
|---|---|---|---|---|---|
| Price(USD) | Turnaround (Week) |
Price(USD) | Turnaround (Week) |
||
| Popular cell line | 2020 | 7-10 | 1780 | 7-9 | 1M cells/vial 2 vials/cell pool |
| Successfully modified cell line (Over 200 types) |
2480 | 8-11 | 1980 | 8-11 | |
| Regular cell line | 2980 | 9-14 | 2480 | 9-13 | |
| Note | Popular cell lines: Hela, HEK-293, A549, HCT116, 4T1, SGC-7901, NCI-H1299 | ||||
| Service | List price(USD) | Promotional price(USD) | |
|---|---|---|---|
| Single cell clone isolation (2 clones) |
Regular cell lines | 500 | 250 |
Adding preferred good-quality serum according to the characteristics of different cells.
Performing multiple optimization and testing of the proportion of medium components.
Significantly increase the monoclonal formation rate up to 30%.
Ubigene has tested this product in some cell lines that are difficult to form colonies (Table 1).
To learn more about Cell Monoclonal Culture Medium>>| Cell line | Morphology | Single-cell clone fomation rate | Common culture medium | EZ-editor™ Cell Monoclonal Culture Medium |
|---|---|---|---|---|
| SK-N-SH | Epithelial-like, adherent | Hard | <1% | 20-30% |
| Huh-7 | Epithelial-like, adherent | Hard | 1-5% | 15-25% |
| Hep G2 | Epithelial-like, adherent | Hard | 4-8% | 20-30% |
| 22RV1 | Epithelial-like, adherent | Hard | 8-12% | 20-30% |
| MDA-MB-231 | Epithelial-like, adherent | Medium | 8-12% | 25-35% |
| THP-1 | Monocyte, suspension | Medium | 15-19% | 25-35% |
| HEK293 | Epithelial-like, adherent | Easy | 16-20% | 32-38% |
Table 1: EZ-editor™ Cell Monoclonal Culture Medium significantly increase the monoclonal formation rateNote: This promotion is limited to scientific research customers such as universities and research institutes
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