Catalog#：YC-C094-Cas9-H
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BAF3 Cell Line was generated by CRISPR-U™ gene-editing technology to stably express Cas9 protein.
| Catalog | YC-C094-Cas9-H | ||
| Cell line | BAF3-CAS9 | Cell Type | Mouse Pro B Cell Line |
| Morphology | Lymphoblast, suspension | Passage ratio | Control the cell density at 2x10^5~4.0x10^5 cells/ml during passaging, and carry out the passage when the cells grow to 8x10^5~1.0x10^6 cells/ml. |
| Culture method | 90%RPMI1640+10%FBS+IL-3 (Mouse)10ng/ml | ||
| Cryopreservation solution | 90%FBS+10%DMSO | ||
| Special Note | The cell culture density needs to be strictly controlled. | ||
| Sample Name | Target Name | Cт Mean | ΔCт |
| BAF3-CAS9 | Cas9 | 19.86832619 | 5.97091389 |
| BAF3-CAS9 | h-actin-B | 13.89741230 | |
| BAF3 | Cas9 | 37.48333359 | 22.66687393 |
| BAF3 | h-actin-B | 14.81645966 |
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