CNE1-CAS9 cell line

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CNE1-CAS9 (Hela-contaminated cells, not for sales) Order

Catalog#

YC-C062-Cas9-H

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Order

Detailed Product Information

Product category

Cas9 Stable Cell Lines

cell name

CNE1-CAS9

Cell Morphology

Endothelial cells, adherent

Passage ratio

1:3~1:5

Species

Human

Expression gene

Cas9

Resistance gene

hygro

Construction method

Lentivirus

Mycoplasma test

negative

Culture method

90%DMEM+10%FBS

Description

Use Cas9 gene expressing lentivirus constructed by Ubigene to infect the CNE1 cell line， screen the cells based on the resistance marker and the optimal antibiotic screening concentration explored in advance, resulting in a CNE1-CAS9 cell line that can maintain long-term, stable and high expression.

Cutting Efficiency Validation

CNE1-CAS9 cell line Product Validation Data（RT-QPCR）

Note: The above figure shows the sequencing peaks of the CNE1-CAS9 stable cell pool which is electroporated by SETD2 gene targeting gRNA plasmid, after 48h antibiotic screening. The red arrow indicates the position where the nested peak appears, which shows that the genotype of the target site is significantly changed due to the cutting. Therefore, it indicates that Cas9 nuclease is successfully expressed.

STR Profile

Loci	STR Info (Sample Cell) Sample Cell Line:CNE1			STR Info (Cell bank) Cell Line:CNE-1
Loci	Allele1	Allele2	Allele3	Allele1	Allele2	Allele3
D5S818	11	12		11	12
D13S317	10	12	13.3	10	12	13.3
D7S820	10	12		10	12
D16S539	9	10		9	10
VWA	14	16		14	16
TH01	6	7	9.0	6	7	9.0
AMEL	X	X		X	X
TPOX	8	12		8	12
CSF1PO	10	11		10	11
D12S391	20	21
FGA	18	21
D2S1338	17	23
D21S11	27	30
D18S51	13	16
D8S1179	12	16
D3S1358	15	18
D6S1043	11	18
PENTAE	17	20
D19S433	13	13
PENTAD	9	12
D1S1656	12	15	16.0

* Hela-contaminated cells, not for sales

Reviewed by ICLAC (ref: 121113, 150506). STR profiling in Chan et al (2008) demonstrated similarity to HeLa, but at less than 80% match. Strong et al (2014) presented additional data in support of this finding, demonstrating that cells carry HPV-18, as previously demonstrated in HeLa cells. The authors speculate that a fusion event may have occurred between HeLa and an unknown cell line; although not proven, current data are consistent with this hypothesis.

Product Advantage

Easy to use：Cas9 cell lines in our cell bank can stably express Cas9 protein. Each Cas9 Stable Cell Line is easy to use and enables gene knockout simply by transfecting gRNA, while transfection of gRNA and donor DNA results in gene knock-in or point mutations
01
High-efficient KO：These Cas9 stable cell lines have been used to construct the KO cell lines for various genes, gene KO efficiency 5-10 times improved.
02
High-quality cell：Selected cells, low passages, good cell condition, high activity, applicable for all kinds of gene-editing experiments.
03

CNE1-CAS9

Catalog#

YC-C062-Cas9-H

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Order

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CNE1-CAS9 cell line

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