HuH-7-CAS9

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HuH-7-CAS9 Order

Catalog#：YC-D001-Cas9-H

Price (USD)：-

Size： 1*10^6

Instruction：

HuH-7 Cell Line was generated by CRISPR-U™ gene-editing technology to stably express Cas9 protein.

Detailed Product Information Recommended Companion Products

Cell Info

Catalog	YC-D001-Cas9-H
Cell line	HuH-7-CAS9	Cell Type	Human Hepatoma Cell Line
Morphology	Epithelial-like, adherent	Passage ratio	1:2~1:3
Culture method	90%DMEM+10% FBS
Cryopreservation solution	50%DMEM+40% FBS+10%DMSO
Special Note

Product Validation Data

RT-QPCR

Sample Name	Target Name	Cт Mean	ΔCт
HuH-7-CAS9	Cas9	20.01689911	3.60826302
HuH-7-CAS9	h-actin-B	16.40863609	3.60826302
HuH-7	Cas9	35.83909988	18.00432014
HuH-7	h-actin-B	17.83477974	18.00432014

Cutting Efficiency Validation

Note: The above figure shows the sequencing peaks of the HuH-7-CAS9 stable cell pool which is electroporated by NONO gene targeting gRNA plasmid, after 48h antibiotic screening. The red arrow indicates the position where the nested peak appears, which shows that the genotype of the target site is significantly changed due to the cutting. Therefore, it indicates that Cas9 nuclease is successfully expressed.

Product Advantage

（1）Easy to use：Cas9 cell lines in our cell bank can stably express Cas9 protein. Each Cas9 Stable Cell Line is easy to use and enables gene knockout simply by transfecting gRNA, while transfection of gRNA and donor DNA results in gene knock-in or point mutations
（2）High-efficient KO：These Cas9 stable cell lines have been used to construct the KO cell lines for various genes, gene KO efficiency 5-10 times improved.
（3）High-quality cell：Selected cells, low passages, good cell condition, high activity, applicable for all kinds of gene-editing experiments.

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