HuH-7-CAS9 cell line

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HuH-7-CAS9 Order

HuH-7-CAS9 cell line
Catalog#
YC-D001-Cas9-H
Size
1*10^6
Transfection Culture Medium
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Datasheet
HuH-7-CAS9 cell line
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Detailed Product Information

Product category
Cas9 Stable Cell Lines
cell name
HuH-7-CAS9
Morphology
Epithelial-like, adherent
Passage ratio
1:2~1:3
Species
Human
Expression gene
Cas9
Resistance gene
hygro
Construction method
Lentivirus
Mycoplasma test
negative
Culture method
90%DMEM+10% FBS
Description
Use Cas9 gene expressing lentivirus constructed by Ubigene to infect the HuH-7 cell line, screen the cells based on the resistance marker and the optimal antibiotic screening concentration explored in advance, resulting in a HuH-7-CAS9 cell line that can maintain long-term, stable and high expression.
Product Validation Data(RT-QPCR)
Sample Name Target Name Cт Mean ΔCт
HuH-7-CAS9 Cas9 20.01689911 3.60826302
HuH-7-CAS9 h-actin-B 16.40863609
HuH-7 Cas9 35.83909988 18.00432014
HuH-7 h-actin-B 17.83477974
Cutting Efficiency Validation
HuH-7-CAS9 cell line Product Validation Data(RT-QPCR)
Note: The above figure shows the sequencing peaks of the HuH-7-CAS9 stable cell pool which is electroporated by NONO gene targeting gRNA plasmid, after 48h antibiotic screening. The red arrow indicates the position where the nested peak appears, which shows that the genotype of the target site is significantly changed due to the cutting. Therefore, it indicates that Cas9 nuclease is successfully expressed.
Loci STR Info (Sample Cell)
Sample Cell Line:HuH-7
STR Info (Cell bank)
Cell Line:HuH-7
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 12121212
D13S317 10111011
D7S820 11111111
D16S539 10101010
VWA 16181618
TH01 7777
AMEL XXXX
TPOX 811811
CSF1PO 11111111
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Product Advantage

  • Easy to use:Cas9 cell lines in our cell bank can stably express Cas9 protein. Each Cas9 Stable Cell Line is easy to use and enables gene knockout simply by transfecting gRNA, while transfection of gRNA and donor DNA results in gene knock-in or point mutations
    01
  • High-efficient KO:These Cas9 stable cell lines have been used to construct the KO cell lines for various genes, gene KO efficiency 5-10 times improved.
    02
  • High-quality cell:Selected cells, low passages, good cell condition, high activity, applicable for all kinds of gene-editing experiments.
    03

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