LS 174T-CAS9 cell line

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LS 174T-CAS9 Order

Catalog#

YC-C088-Cas9-H

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Order

Detailed Product Information

Product category

Cas9 Stable Cell Lines

cell name

LS 174T-CAS9

Cell Morphology

Epithelial-like,adherent

Passage ratio

1:2~1:4

Species

Human

Expression gene

Cas9

Resistance gene

hygro

Construction method

Lentivirus

Mycoplasma test

negative

Culture method

90%DMEM+10% FBS

Description

Use Cas9 gene expressing lentivirus constructed by Ubigene to infect the LS 174T cell line， screen the cells based on the resistance marker and the optimal antibiotic screening concentration explored in advance, resulting in a LS 174T-CAS9 cell line that can maintain long-term, stable and high expression.

Cutting Efficiency Validation

LS 174T-CAS9 cell line Product Validation Data（RT-QPCR）

Note: The above figure shows the sequencing peaks of the LS-174T-CAS9 stable cell pool which is electroporated by NONO gene targeting gRNA plasmid, after 48h antibiotic screening. The red arrow indicates the position where the nested peak appears, which shows that the genotype of the target site is significantly changed due to the cutting. Therefore, it indicates that Cas9 nuclease is successfully expressed.

STR Profile

Loci	STR Info (Sample Cell) Sample Cell Line:LS 174T			STR Info (Cell bank) Cell Line:LS174T
Loci	Allele1	Allele2	Allele3	Allele1	Allele2	Allele3
D5S818	11	16		11	14	15.0
D13S317	10	10		10	10
D7S820	11	11		11	11
D16S539	11	13		11	13
VWA	15	16	17.0	15	17	18.0
TH01	6	7		6	7
AMEL	X	X		X	X
TPOX	8	9		8	9
CSF1PO	10	13		10	14
D12S391	16	20
FGA	20	21	22.0
D2S1338	18	22
D21S11	29	30	31.0
D18S51	11	13
D8S1179	12	16
D3S1358	15	17
D6S1043	12	13	14.0
PENTAE	15	16
D19S433	14	15
PENTAD	10	10
D1S1656	12	13	18.3

* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Product Advantage

Easy to use：Cas9 cell lines in our cell bank can stably express Cas9 protein. Each Cas9 Stable Cell Line is easy to use and enables gene knockout simply by transfecting gRNA, while transfection of gRNA and donor DNA results in gene knock-in or point mutations
01
High-efficient KO：These Cas9 stable cell lines have been used to construct the KO cell lines for various genes, gene KO efficiency 5-10 times improved.
02
High-quality cell：Selected cells, low passages, good cell condition, high activity, applicable for all kinds of gene-editing experiments.
03

LS 174T-CAS9

Catalog#

YC-C088-Cas9-H

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Order

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LS 174T-CAS9 cell line

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