MS751-CAS9 cell line

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MS751-CAS9 Order

Catalog#

YC-C123-Cas9-H

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Datasheet

Order

Detailed Product Information

Product category

Cas9 Stable Cell Lines

cell name

MS751-CAS9

Cell Morphology

Epithelial-like, adherent

Passage ratio

1:2-1:3

Species

Human

Expression gene

Cas9

Resistance gene

hygro

Construction method

Lentivirus

Mycoplasma test

negative

Culture method

88%MEM+10%FBS+1%NEAA+1%Sodium Pyruvate

Description

Use Cas9 gene expressing lentivirus constructed by Ubigene to infect the MS751 cell line， screen the cells based on the resistance marker and the optimal antibiotic screening concentration explored in advance, resulting in a MS751-CAS9 cell line that can maintain long-term, stable and high expression.

Product Validation Data（RT-QPCR）

Sample Name	Target Name	Cт Mean	ΔCт
MS751-CAS9	Cas9	15.68006420	1.25605297
MS751-CAS9	β-actin	14.42401123	1.25605297
MS751	Cas9	34.88079834	17.69993019
MS751	β-actin	17.18086815	17.69993019

Cutting Efficiency Validation

MS751-CAS9 cell line Product Validation Data（RT-QPCR）

Note: The above figure shows the sequencing peaks of the MS751-CAS9 stable cell pool which is electroporated by KMT2D gene targeting gRNA plasmid, after 48h antibiotic screening. The red arrow indicates the position where the nested peak appears, which shows that the genotype of the target site is significantly changed due to the cutting. Therefore, it indicates that Cas9 nuclease is successfully expressed.

STR Profile

Loci	STR Info (Sample Cell) Sample Cell Line:MS751			STR Info (Cell bank) Cell Line:MS751
Loci	Allele1	Allele2	Allele3	Allele1	Allele2	Allele3
D5S818	12	12		12	12
D13S317	12	12		12	12
D7S820	9	11		9	11
D16S539	11	11		11	11
VWA	16	16		16	16
TH01	6	6		6	6
AMEL	X	X		X	X
TPOX	8	8		8	8
CSF1PO	11	12		11	11
D12S391	15	22
FGA	20	20
D2S1338	22	22
D21S11	29	30
D18S51	12	12
D8S1179	14	14
D3S1358	19	19
D6S1043	11	12
PENTAE	12	12
D19S433	16	16
PENTAD	9	9
D1S1656	12	17

* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Product Advantage

Easy to use：Cas9 cell lines in our cell bank can stably express Cas9 protein. Each Cas9 Stable Cell Line is easy to use and enables gene knockout simply by transfecting gRNA, while transfection of gRNA and donor DNA results in gene knock-in or point mutations
01
High-efficient KO：These Cas9 stable cell lines have been used to construct the KO cell lines for various genes, gene KO efficiency 5-10 times improved.
02
High-quality cell：Selected cells, low passages, good cell condition, high activity, applicable for all kinds of gene-editing experiments.
03

MS751-CAS9

Catalog#

YC-C123-Cas9-H

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Order

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MS751-CAS9 cell line

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