PC-3M-CAS9 cell line

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PC-3M-CAS9 Order

PC-3M-CAS9 cell line
Catalog#
YC-C099-Cas9-H
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
PC-3M-CAS9 cell line
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Detailed Product Information

Product category
Cas9 Stable Cell Lines
cell name
PC-3M-CAS9
Cell Morphology
Epithelial-like, adherent
Passage ratio
1:2-1:4
Species
Human
Expression gene
Cas9
Resistance gene
hygro
Construction method
Lentivirus
Mycoplasma test
negative
Culture method
90%RPMI-1640+10%FBS
Description
Use Cas9 gene expressing lentivirus constructed by Ubigene to infect the PC-3M cell line， screen the cells based on the resistance marker and the optimal antibiotic screening concentration explored in advance, resulting in a PC-3M-CAS9 cell line that can maintain long-term, stable and high expression.
Cutting Efficiency Validation
PC-3M-CAS9 cell line Product Validation Data（RT-QPCR）
Note: The above figure shows the sequencing peaks of the PC-3M-CAS9 stable cell pool which is electroporated by SDHB gene targeting gRNA plasmid, after 48h antibiotic screening. The red arrow indicates the position where the nested peak appears, which shows that the genotype of the target site is significantly changed due to the cutting. Therefore, it indicates that Cas9 nuclease is successfully expressed.
STR Profile
Click to download the STR appraisal report
Loci STR Info (Sample Cell)
Sample Cell Line:PC-3M		 STR Info (Cell bank)
Cell Line:PC-3M
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 13131313
D13S317 11111111
D7S820 811811
D16S539 11111111
VWA 17171717
TH01 6767
AMEL XXXX
TPOX 8989
CSF1PO 11111111
D12S391 2121
FGA 2424
D2S1338 1820
D21S11 2931.2
D18S51 1415
D8S1179 1313
D3S1358 1616
D6S1043 1418
PENTAE 1017
D19S433 1414
PENTAD 99
D1S1656 1216
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Product Advantage

  • Easy to use：Cas9 cell lines in our cell bank can stably express Cas9 protein. Each Cas9 Stable Cell Line is easy to use and enables gene knockout simply by transfecting gRNA, while transfection of gRNA and donor DNA results in gene knock-in or point mutations
    01
  • High-efficient KO：These Cas9 stable cell lines have been used to construct the KO cell lines for various genes, gene KO efficiency 5-10 times improved.
    02
  • High-quality cell：Selected cells, low passages, good cell condition, high activity, applicable for all kinds of gene-editing experiments.
    03
PC-3M-CAS9 cell line
PC-3M-CAS9
Catalog#
YC-C099-Cas9-H
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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