RKO-CAS9 cell line

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RKO-CAS9 Order

RKO-CAS9 cell line
Catalog#
YC-C002-Cas9-H
Size
1*10^6
Transfection Culture Medium
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Datasheet
RKO-CAS9 cell line
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Detailed Product Information

Product category
Cas9 Stable Cell Lines
cell name
RKO-CAS9
Morphology
Epithelial-like, adherent
Passage ratio
1:2~1:4
Species
Human
Expression gene
Cas9
Resistance gene
hygro
Construction method
Lentivirus
Mycoplasma test
negative
Culture method
90%DMEM+10%FBS
Description
Use Cas9 gene expressing lentivirus constructed by Ubigene to infect the RKO cell line, screen the cells based on the resistance marker and the optimal antibiotic screening concentration explored in advance, resulting in a RKO-CAS9 cell line that can maintain long-term, stable and high expression.
Product Validation Data(RT-QPCR)
Sample Name Target Name Cт Mean ΔCт
RKO-CAS9 Cas9 21.27658653 3.43598557
RKO-CAS9 h-actin-B 17.84060097
RKO Cas9 35.33678818 17.99855232
RKO h-actin-B 17.33823586
Cutting Efficiency Validation
RKO-CAS9 cell line Product Validation Data(RT-QPCR)
Note: The above figure shows the sequencing peaks of the RKO-CAS9 stable cell pool which is electroporated by NONO gene targeting gRNA plasmid, after 48h antibiotic screening. The red arrow indicates the position where the nested peak appears, which shows that the genotype of the target site is significantly changed due to the cutting. Therefore, it indicates that Cas9 nuclease is successfully expressed.
Loci STR Info (Sample Cell)
Sample Cell Line:RKO
STR Info (Cell bank)
Cell Line:RKO
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 11131113
D13S317 811811
D7S820 810810
D16S539 13131213
VWA 16221622
TH01 610610
AMEL XXXX
TPOX 11111111
CSF1PO 810810
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Product Advantage

  • Easy to use:Cas9 cell lines in our cell bank can stably express Cas9 protein. Each Cas9 Stable Cell Line is easy to use and enables gene knockout simply by transfecting gRNA, while transfection of gRNA and donor DNA results in gene knock-in or point mutations
    01
  • High-efficient KO:These Cas9 stable cell lines have been used to construct the KO cell lines for various genes, gene KO efficiency 5-10 times improved.
    02
  • High-quality cell:Selected cells, low passages, good cell condition, high activity, applicable for all kinds of gene-editing experiments.
    03

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