THP-1-CAS9

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THP-1-CAS9 Order

Catalog#：YC-D011-Cas9-H

Price (USD)：-

Size： 1*10^6

Instruction：

THP-1 Cell Line was generated by CRISPR-U™ gene-editing technology to stably express Cas9 protein.

Detailed Product Information Recommended Companion Products

Cell Info

Catalog	YC-D011-Cas9-H
Cell line	THP-1-CAS9	Cell Type	Human Ovarian Granulosa Cell Line
Morphology	Monocyte, suspension	Passage ratio	Control the cell density at 2x10^5~4.0x10^5 cells/ml during passaging, and carry out the passage when the cells grow to 8x10^5~1.0x10^6 cells/ml.
Culture method	90%RPMI-1640+10%FBS
Cryopreservation solution	90%FBS+10%DMSO
Special Note	The cell culture density needs to be strictly controlled. The cell viability after thawing is relatively low, it is recommended to use Australia-sourced FBS for thawing or increase the FBS ratio to 20%. When the cells grow normally and can be passaged after 2 passages, the cells can be cultured using the normal culture method.

Product Validation Data

RT-QPCR

Sample Name	Target Name	Cт Mean	ΔCт
THP-1-CAS9	Cas9	18.14743614	4.86366844
THP-1-CAS9	h-actin-B	13.28376770	4.86366844
THP-1	Cas9	34.46055222	21.80498600
THP-1	h-actin-B	12.65556622	21.80498600

Cutting Efficiency Validation

Note: The above figure shows the sequencing peaks of the THP-1-CAS9 stable cell pool which is electroporated by NONO gene targeting gRNA plasmid, after 48h antibiotic screening. The red arrow indicates the position where the nested peak appears, which shows that the genotype of the target site is significantly changed due to the cutting. Therefore, it indicates that Cas9 nuclease is successfully expressed.

Product Advantage

（1）Easy to use：Cas9 cell lines in our cell bank can stably express Cas9 protein. Each Cas9 Stable Cell Line is easy to use and enables gene knockout simply by transfecting gRNA, while transfection of gRNA and donor DNA results in gene knock-in or point mutations
（2）High-efficient KO：These Cas9 stable cell lines have been used to construct the KO cell lines for various genes, gene KO efficiency 5-10 times improved.
（3）High-quality cell：Selected cells, low passages, good cell condition, high activity, applicable for all kinds of gene-editing experiments.

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