U251MG-CAS9 cell line

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U251MG-CAS9 Order

Catalog#

YC-D015-Cas9-H

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Datasheet

Order

Detailed Product Information

Product category

Cas9 Stable Cell Lines

cell name

U251MG-CAS9

Cell Morphology

Epithelial-like, adherent

Passage ratio

1:2~1:4

Species

Human

Expression gene

Cas9

Resistance gene

hygro

Construction method

Lentivirus

Mycoplasma test

negative

Culture method

90% DMEM+10%FBS

Description

Use Cas9 gene expressing lentivirus constructed by Ubigene to infect the U251MG cell line， screen the cells based on the resistance marker and the optimal antibiotic screening concentration explored in advance, resulting in a U251MG-CAS9 cell line that can maintain long-term, stable and high expression.

Product Validation Data（RT-QPCR）

Sample Name	Target Name	Cт Mean	ΔCт
U251MG-CAS9	Cas9	14.25889301	-1.58300591
U251MG-CAS9	β-actin	15.84189892	-1.58300591
U251MG	Cas9	35.44923401	19.64437771
U251MG	β-actin	15.80485630	19.64437771

Cutting Efficiency Validation

U251MG-CAS9 cell line Product Validation Data（RT-QPCR）

Note: The above figure shows the sequencing peaks of the U251MG-CAS9 stable cell pool which is electroporated by SLC30A9 gene targeting gRNA plasmid, after 48h antibiotic screening. The red arrow indicates the position where the nested peak appears, which shows that the genotype of the target site is significantly changed due to the cutting. Therefore, it indicates that Cas9 nuclease is successfully expressed.

STR Profile

Loci	STR Info (Sample Cell) Sample Cell Line:U251MG			STR Info (Cell bank) Cell Line:KNS-89
Loci	Allele1	Allele2	Allele3	Allele1	Allele2	Allele3
D5S818	11	12		11	12
D13S317	10	11		10	11
D7S820	10	12		10	12
D16S539	12	12		12	12
VWA	16	18		16	18
TH01	9.3	9.3		9.3	9.3
AMEL	X	X		X	X
TPOX	8	8		8	8
CSF1PO	11	12		11	12
D12S391	17	22
FGA	21	25
D2S1338	24	24
D21S11	29	29
D18S51	13	13
D8S1179	13	15
D3S1358	16	16
D6S1043	12	12
PENTAE	7	10
D19S433	13	15
PENTAD	12	12
D1S1656	16	17.3

* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Product Advantage

Easy to use：Cas9 cell lines in our cell bank can stably express Cas9 protein. Each Cas9 Stable Cell Line is easy to use and enables gene knockout simply by transfecting gRNA, while transfection of gRNA and donor DNA results in gene knock-in or point mutations
01
High-efficient KO：These Cas9 stable cell lines have been used to construct the KO cell lines for various genes, gene KO efficiency 5-10 times improved.
02
High-quality cell：Selected cells, low passages, good cell condition, high activity, applicable for all kinds of gene-editing experiments.
03

U251MG-CAS9

Catalog#

YC-D015-Cas9-H

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Order

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U251MG-CAS9 cell line

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