HT-29-EGFP cell line

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HT-29-EGFP Order

HT-29-EGFP cell line
Catalog#
YC-C008-EGFP-P
Size
1*10^6
Transfection Culture Medium
Transfection Culture Medium-5A
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Datasheet
HT-29-EGFP cell line
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Detailed Product Information

Product category
EGFP Stable Cell Lines
cell name
HT-29-EGFP
Cell Morphology
Epithelial-like, adherent
Passage ratio
1:2~1:4
Species
Human
Reporter gene
EGFP
Resistance gene
puro
Construction method
Lentivirus
Mycoplasma test
negative
Culture method
90%McCOY's 5A+10% FBS
STR Profile
Click to download the STR appraisal report
Loci STR Info (Sample Cell)
Sample Cell Line:HT-29		 STR Info (Cell bank)
Cell Line:HT-29
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 11121112
D13S317 11121112
D7S820 10101010
D16S539 11121112
VWA 17191719
TH01 6969
AMEL XXXX
TPOX 8989
CSF1PO 11121112
D12S391 18.321
FGA 2022
D2S1338 1923
D21S11 2930
D18S51 1313
D8S1179 1010
D3S1358 1517
D6S1043 1214
PENTAE 1416
D19S433 1414.2
PENTAD 1113
D1S1656 1516
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.
EGFP validation
HT-29-EGFP cell line EGFP validation

Instruction

Use EGFP gene expressing lentivirus constructed by Ubigene to infect the HT-29 cell line， screen the cells based on the puromycin resistance marker and the optimal antibiotic screening concentration explored in advance, resulting in a HT-29-EGFP cell line that can maintain long-term, stable and high expression.
HT-29-EGFP cell line
HT-29-EGFP
Catalog#
YC-C008-EGFP-P
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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