SK-BR-3-EGFP cell line

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SK-BR-3-EGFP Order

SK-BR-3-EGFP cell line
Catalog#
YC-C048-EGFP-P
Size
1*10^6
Transfection Culture Medium
Transfection Culture medium-A
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Datasheet
SK-BR-3-EGFP cell line
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Detailed Product Information

Product category
EGFP Stable Cell Lines
cell name
SK-BR-3-EGFP
Cell Morphology
Epithelial-like, adherent
Passage ratio
1:2~1:3
Species
Human
Reporter gene
EGFP
Resistance gene
puro
Construction method
Lentivirus
Mycoplasma test
negative
Culture method
90%McCOY's 5A+10%FBS
Accession
CVCL_C8WI(Cellosaurus)
ExPASy Cellosaurus Database
Is a valuable online resource that compiles detailed information on over 100,000 cell lines from reputable cell banks such as ATCC, ECACC, and DSMZ. The database provides information including descriptions, origins, and characteristics of the cell lines, with broad reference value and scientific reliability. Additionally, the database provides information on cell line authentication, STR profiles, and potential contamination risks, helping researchers ensure the trustworthiness of the cell lines used in their experiments.
STR Profile
Click to download the STR appraisal report
Loci STR Info (Sample Cell)
Sample Cell Line:SK-BR-3		 STR Info (Cell bank)
Cell Line:SK-BR-3
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 912912
D13S317 11121112
D7S820 99912
D16S539 9999
VWA 17171717
TH01 8989
AMEL XXXX
TPOX 811811
CSF1PO 12121212
D12S391 1820
FGA 2020
D2S1338 2025
D21S11 3030.2
D18S51 1013
D8S1179 1212
D3S1358 1717
D6S1043 1119
PENTAE 1011
D19S433 1414
PENTAD 912
D1S1656 1117.3
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Instruction

Use EGFP gene expressing lentivirus constructed by Ubigene to infect the SK-BR-3 cell line， screen the cells based on the puromycin resistance marker and the optimal antibiotic screening concentration explored in advance, resulting in a SK-BR-3-EGFP cell line that can maintain long-term, stable and high expression.
SK-BR-3-EGFP cell line
SK-BR-3-EGFP
Catalog#
YC-C048-EGFP-P
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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