ADPRS Knockout cell line (HCC1806)

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ADPRS Knockout cell line (HCC1806) Order

ADPRS Knockout cell line (HCC1806)
Catalog#
YKO-H1057
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
ADPRS Knockout cell line (HCC1806)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
ADPRS Knockout cell line (HCC1806)
Cell Morphology
Endothelial cells, adherent
Passage ratio
1:2~1:4
Species
Human
Gene Name
ADPRS
Gene ID
54936
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
90%RPMI-1640+10% FBS
Accession
CVCL_D8GW(Cellosaurus)
ExPASy Cellosaurus Database
Is a valuable online resource that compiles detailed information on over 100,000 cell lines from reputable cell banks such as ATCC, ECACC, and DSMZ. The database provides information including descriptions, origins, and characteristics of the cell lines, with broad reference value and scientific reliability. Additionally, the database provides information on cell line authentication, STR profiles, and potential contamination risks, helping researchers ensure the trustworthiness of the cell lines used in their experiments.
Target region
Knockout region: Exon 1 (based on transcript ADPRS-201)
Validation: PCR+Sanger Sequencing
ADPRS Knockout cell line (HCC1806) Target region
STR Profile
Click to download the STR appraisal report
Loci STR Info (Sample Cell)
Sample Cell Line:HCC1806		 STR Info (Cell bank)
Cell Line:HCC 1806
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 13131313
D13S317 11111111
D7S820 10121012
D16S539 10101010
VWA 16181618
TH01 8888
AMEL XXXX
TPOX 8889
CSF1PO 12121212
D12S391 1921
FGA 2525
D2S1338 1717
D21S11 2929
D18S51 1616
D8S1179 1415
D3S1358 1616
D6S1043 1212
PENTAE 1212
D19S433 1414
PENTAD 1515
D1S1656 15.315.3
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the ADPRS gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the HCC1806 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, ADPRS gene knockout ADPRS Knockout cell line (HCC1806) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
ADPRS Knockout cell line (HCC1806)
ADPRS Knockout cell line (HCC1806)
Catalog#
YKO-H1057
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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