Atg5 Knockout cell line (HT-22)

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Atg5 Knockout cell line (HT-22) Order

Atg5 Knockout cell line (HT-22)
Catalog#
YKO-M197
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Atg5 Knockout cell line (HT-22)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
Atg5 Knockout cell line (HT-22)
Cell Morphology
Neurocyte like，adherent
Passage ratio
1：3-1：5
Species
Mouse
Gene Name
Atg5
Gene ID
11793
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
90%DMEM+10%FBS
Accession
CVCL_D9WN(Cellosaurus)
ExPASy Cellosaurus Database
Is a valuable online resource that compiles detailed information on over 100,000 cell lines from reputable cell banks such as ATCC, ECACC, and DSMZ. The database provides information including descriptions, origins, and characteristics of the cell lines, with broad reference value and scientific reliability. Additionally, the database provides information on cell line authentication, STR profiles, and potential contamination risks, helping researchers ensure the trustworthiness of the cell lines used in their experiments.
Target region
Knockout region: Exon 2 (based on transcript Atg5-201)
Validation: PCR+Sanger Sequencing
Atg5 Knockout cell line (HT-22) Target region
STR Profile
Click to download the STR appraisal report
Loci STR Info (Sample Cell)
Sample Cell Line:HT-22		 STR Info (Cell bank)
Cell Line:HT22
Allele1Allele2Allele3Allele4Allele1Allele2Allele3
18-3 17.019.017.019.0
4-2 18.319.320.318.319.320.3
6-7 12.012.0
19-2 11.012.011.012.0
1-2 13.017.013.017.0
7-1 29.029.0
8-1 15.015.0
1-1 10.011.010.011.0
3-2 14.015.014.015.0
2-1 9.09.0
15-3 20.320.3
6-4 16.015.3
13-1 16.216.2
11-2 15.017.018.015.017.018.0
17-2 13.014.012.013.014.0
12-1 20.020.0
5-5 13.014.015.013.014.015.0
X-1 25.025.0
TH01
D5S818
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the Atg5 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the HT-22 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, Atg5 gene knockout Atg5 Knockout cell line (HT-22) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
Atg5 Knockout cell line (HT-22)
Atg5 Knockout cell line (HT-22)
Catalog#
YKO-M197
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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