CLEC3B Knockout cell line (HMC3)

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CLEC3B Knockout cell line (HMC3) Order

Catalog#

YKO-H1120

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Order

Detailed Product Information

Product category

KO Cell Lines

cell name

CLEC3B Knockout cell line (HMC3)

Cell Morphology

Epithelial-like, adherent

Passage ratio

1：2~1：3

Species

Human

Gene Name

CLEC3B

Gene ID

7123

Gene Information

NCBI

Construction method

Electroporation/Lentivirus

Mycoplasma test

negative

Culture method

90%MEM+10%FBS

Accession

CVCL_D9WB(Cellosaurus)

Target region

Knockout region: Exon 2 (based on transcript CLEC3B-201)
Validation: PCR+Sanger Sequencing

STR Profile

Loci	STR Info (Sample Cell) Sample Cell Line:HMC3			STR Info (Cell bank) Cell Line:HMC3
Loci	Allele1	Allele2	Allele3	Allele1	Allele2	Allele3
D5S818	11	12		11	12
D13S317	11	11		11	11
D7S820	9	11		9	11
D16S539	12	13		12	13
VWA	17	19		17	19
TH01	6	6		6	6
AMEL	X	X		X	X
TPOX	8	9		8	9
CSF1PO	10	11		10	11
D12S391	16	21
FGA	21	25
D2S1338	17	25
D21S11	30	31.2
D18S51	18	18
D8S1179	13	14
D3S1358	16	18
D6S1043	11	11
PENTAE	7	13
D19S433	15	15.2
PENTAD	10	14
D1S1656	12	13

* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the CLEC3B gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the HMC3 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, CLEC3B gene knockout CLEC3B Knockout cell line (HMC3) will be expanded and cryopreserved.

Knockout Cell Line Work Flow and Validatio

CLEC3B Knockout cell line (HMC3)

Catalog#

YKO-H1120

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Order

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CLEC3B Knockout cell line (HMC3)

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