Casp8 Knockout cell line (MLE-12)

Casp8 Knockout cell line (MLE-12) Order

Catalog#

YKO-M242

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Order

Detailed Product Information

Product category

KO Cell Lines

cell name

Casp8 Knockout cell line（MLE-12）

Cell Morphology

Epithelial-like, adheren

Passage ratio

1：2-1：3

Species

Mouse

Gene Name

Casp8

Gene ID

12370

Gene Information

NCBI

Construction method

Electroporation/Lentivirus

Mycoplasma test

negative

Culture method

Speciality Media

Target region

Knockout region: Exon 3 (based on transcript Casp8-204)
Validation: PCR+Sanger Sequencing

STR Profile

Loci	STR Info (Sample Cell) Sample Cell Line:MLE-12				STR Info (Cell bank) Cell Line:MLE-12
Loci	Allele1	Allele2	Allele3	Allele4	Allele1	Allele2	Allele3
18-3	17.0				156.39 【17】
4-2	19.3				233.94 【19.3】
6-7	12.0				334.52 【12】
19-2	12.0
1-2	13.0
7-1	29.0
8-1	15.0
1-1	10.0
3-2	14.0
2-1	9.0
15-3	20.3				192.1 【20.3】
6-4	16.0				290.93 【15.3】
13-1	15.2
11-2	15.0
17-2	15.0
12-1	20.0				242 【20】
5-5	14.0				335.92 【14】
X-1	26.0				405.05 【25.3】
TH01
D5S818

* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the Casp8 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the MLE-12 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, Casp8 gene knockout Casp8 Knockout cell line（MLE-12） will be expanded and cryopreserved.

Knockout Cell Line Work Flow and Validatio

Casp8 Knockout cell line (MLE-12)

Catalog#

YKO-M242

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Order

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