FGF14 Knockout cell line (H9)

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FGF14 Knockout cell line (H9) Order

FGF14 Knockout cell line (H9)
Catalog#
YKO-H1005
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
FGF14 Knockout cell line (H9)
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Detailed Product Information

Product category
KO Cell Lines
cell name
FGF14 Knockout cell line（H9）
Cell Morphology
Spherical clone
Passage ratio
1:6-1:8
Species
Human
Gene Name
FGF14
Gene ID
2259
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
EZ-Stem™ Cell Culture Medium
Accession
CVCL_D8GC(Cellosaurus)
ExPASy Cellosaurus Database
Is a valuable online resource that compiles detailed information on over 100,000 cell lines from reputable cell banks such as ATCC, ECACC, and DSMZ. The database provides information including descriptions, origins, and characteristics of the cell lines, with broad reference value and scientific reliability. Additionally, the database provides information on cell line authentication, STR profiles, and potential contamination risks, helping researchers ensure the trustworthiness of the cell lines used in their experiments.
Target region
Knockout region: Exon 2 (based on transcript FGF14-202)
Validation: PCR+Sanger Sequencing
FGF14 Knockout cell line (H9) Target region
STR Profile
Click to download the STR appraisal report
Loci STR Info (Sample Cell)
Sample Cell Line:H9		 STR Info (Cell bank)
Cell Line:WAe009-A-5
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 11121112
D13S317 9999
D7S820 911911
D16S539 12131213
VWA 17171717
TH01 9.39.39.39.3
AMEL XXXX
TPOX 10111011
CSF1PO 11111111
D12S391 1519
FGA 2628
D2S1338 1824
D21S11 3030
D18S51 1313
D8S1179 814
D3S1358 1316
D6S1043 1219
PENTAE 1114
D19S433 1215
PENTAD 913
D1S1656 1213
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the FGF14 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the H9 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, FGF14 gene knockout FGF14 Knockout cell line（H9） will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
FGF14 Knockout cell line (H9)
FGF14 Knockout cell line (H9)
Catalog#
YKO-H1005
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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