FMR1 Knockout cell line (SH-SY5Y)

FMR1 Knockout cell line (SH-SY5Y) Order

Catalog#

YKO-H1149

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Order

Detailed Product Information

Product category

KO Cell Lines

cell name

FMR1 Knockout cell line（SH-SY5Y）

Cell Morphology

Epithelial-like, adherent

Passage ratio

1:2-1:3

Species

Human

Gene Name

FMR1

Gene ID

2332

Gene Information

NCBI

Construction method

Electroporation/Lentivirus

Mycoplasma test

negative

Culture method

90%DMEM+10% FBS

Target region

Knockout region: Exon 7 (based on transcript FMR1-205)
Validation: PCR+Sanger Sequencing

STR Profile

Loci	STR Info (Sample Cell) Sample Cell Line:SH-SY5Y			STR Info (Cell bank) Cell Line:SH-SY5Y
Loci	Allele1	Allele2	Allele3	Allele1	Allele2	Allele3
D5S818	12	12		12	12
D13S317	11	11		11	11
D7S820	7	10		7	10
D16S539	8	13		8	13
VWA	14	18		14	18
TH01	7	10		7	10
AMEL	X	X		X	X
TPOX	8	11		8	11
CSF1PO	11	11		11	11
D12S391	18	22
FGA	23.2	24
D2S1338	17	19
D21S11	31	31.2
D18S51	13	16
D8S1179	15	15
D3S1358	15	16
D6S1043	12	18
PENTAE	7	11
D19S433	13	14
PENTAD	10	12
D1S1656	12	12

* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the FMR1 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the SH-SY5Y cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, FMR1 gene knockout FMR1 Knockout cell line（SH-SY5Y） will be expanded and cryopreserved.

Knockout Cell Line Work Flow and Validatio

FMR1 Knockout cell line (SH-SY5Y)

Catalog#

YKO-H1149

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Order

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