GPX4 Knockout cell line (HCT 116)

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GPX4 Knockout cell line (HCT 116) Order

GPX4 Knockout cell line (HCT 116)
Catalog#
YKO-KP248
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
GPX4 Knockout cell line (HCT 116)
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Detailed Product Information

Product category
KO Cell Lines
cell name
GPX4 Knockout cell line (HCT 116)
Morphology
Epithelial-like, adherent
Passage ratio
1:2~1:4
Species
Human
Gene Name
GPX4
Gene ID
2879
Gene Information
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
90%McCOY's 5A+10% FBS

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the GPX4 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the HCT 116 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, GPX4 gene knockout GPX4 Knockout cell line (HCT 116) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
GPX4 Knockout cell line (HCT 116)
GPX4 Knockout cell line (HCT 116)
Catalog#
YKO-KP248
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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