ITCH Knockout cell line (HEK293)

ITCH Knockout cell line (HEK293) Order

Catalog#

YKO-HS5757

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Order

Detailed Product Information

Product category

KO Cell Lines

cell name

ITCH Knockout cell line (HEK293)

Cell Morphology

Epithelial-like, adherent

Passage ratio

1:3~1:6

Species

Human

Gene Name

ITCH

Gene ID

83737

Gene Information

NCBI

Construction method

Electroporation/Lentivirus

Mycoplasma test

negative

Culture method

90%DMEM+10% FBS

Target region

Knockout region: Exon 6 (based on transcript ITCH-202)
Validation: PCR+Sanger Sequencing

STR Profile

Loci	STR Info (Sample Cell) Sample Cell Line:HEK293			STR Info (Cell bank) Cell Line:HEK-293.2sus
Loci	Allele1	Allele2	Allele3	Allele1	Allele2	Allele3
D5S818	8	8		8	8
D13S317	12	14		12	14
D7S820	11	12		11	12
D16S539	9	13		9	13
VWA	16	19		16	19
TH01	7	9.3		7	9.3
AMEL	X	X		X	X
TPOX	11	11		11	11
CSF1PO	12	12		12	12
D12S391	19	21
FGA	23	23
D2S1338	19	19
D21S11	28	30.2
D18S51	17	18
D8S1179	12	14
D3S1358	15	17
D6S1043	11	11
PENTAE	7	15
D19S433	18	18
PENTAD	9	10
D1S1656	15	17.3

* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the ITCH gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the HEK293 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, ITCH gene knockout ITCH Knockout cell line (HEK293) will be expanded and cryopreserved.

Knockout Cell Line Work Flow and Validatio

ITCH Knockout cell line (HEK293)

Catalog#

YKO-HS5757

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Order

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