JAM2 Knockout cell line (HCMEC/D3)

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JAM2 Knockout cell line (HCMEC/D3) Order

JAM2 Knockout cell line (HCMEC/D3)
Catalog#
YKO-H1098
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
JAM2 Knockout cell line (HCMEC/D3)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
JAM2 Knockout cell line (HCMEC/D3)
Morphology
Epithelial-like,?adherent
Passage ratio
1:3~1:4
Species
Human
Gene Name
JAM2
Gene ID
58494
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
Special media
Accession
CVCL_D8GX(Cellosaurus)
ExPASy Cellosaurus Database
Is a valuable online resource that compiles detailed information on over 100,000 cell lines from reputable cell banks such as ATCC, ECACC, and DSMZ. The database provides information including descriptions, origins, and characteristics of the cell lines, with broad reference value and scientific reliability. Additionally, the database provides information on cell line authentication, STR profiles, and potential contamination risks, helping researchers ensure the trustworthiness of the cell lines used in their experiments.
Target region
Knockout region: Exon 1 (based on transcript JAM2-206)
Validation: PCR+Sanger Sequencing
JAM2 Knockout cell line (HCMEC/D3) Target region
STR Profile
Click to download the STR appraisal report
Loci STR Info (Sample Cell)
Sample Cell Line:HCMEC/D3		 STR Info (Cell bank)
Cell Line:hCMEC/D3
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 11111111
D13S317 11141114
D7S820 10121012
D16S539 912912
VWA 14171417
TH01 7979
AMEL XXXX
TPOX 10111011
CSF1PO 10121012
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the JAM2 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the HCMEC/D3 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, JAM2 gene knockout JAM2 Knockout cell line (HCMEC/D3) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
JAM2 Knockout cell line (HCMEC/D3)
JAM2 Knockout cell line (HCMEC/D3)
Catalog#
YKO-H1098
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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