KMT2D Knockout cell line (MS751)

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KMT2D Knockout cell line (MS751) Order

KMT2D Knockout cell line (MS751)
Catalog#
YKO-H766
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
KMT2D Knockout cell line (MS751)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
KMT2D Knockout cell line (MS751)
Cell Morphology
Epithelial-like, adherent
Passage ratio
1:2-1:3
Species
Human
Gene Name
KMT2D
Gene ID
8085
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
88%MEM+10%FBS+1%NEAA+1%Sodium Pyruvate
Accession
CVCL_E0ZB(Cellosaurus)
ExPASy Cellosaurus Database
Is a valuable online resource that compiles detailed information on over 100,000 cell lines from reputable cell banks such as ATCC, ECACC, and DSMZ. The database provides information including descriptions, origins, and characteristics of the cell lines, with broad reference value and scientific reliability. Additionally, the database provides information on cell line authentication, STR profiles, and potential contamination risks, helping researchers ensure the trustworthiness of the cell lines used in their experiments.
Target region
Knockout region: Exon 4 (based on transcript KMT2D-201)
Validation: PCR+Sanger Sequencing
KMT2D Knockout cell line (MS751) Target region
STR Profile
Click to download the STR appraisal report
Loci STR Info (Sample Cell)
Sample Cell Line:MS751		 STR Info (Cell bank)
Cell Line:MS751
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 12121212
D13S317 12121212
D7S820 911911
D16S539 11111111
VWA 16161616
TH01 6666
AMEL XXXX
TPOX 8888
CSF1PO 11121111
D12S391 1522
FGA 2020
D2S1338 2222
D21S11 2930
D18S51 1212
D8S1179 1414
D3S1358 1919
D6S1043 1112
PENTAE 1212
D19S433 1616
PENTAD 99
D1S1656 1217
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the KMT2D gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the MS751 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, KMT2D gene knockout KMT2D Knockout cell line (MS751) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
KMT2D Knockout cell line (MS751)
KMT2D Knockout cell line (MS751)
Catalog#
YKO-H766
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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